应用ISSR分子标记技术对本溪草河口林场的红松(Pinus koraiensis)人工林两个种群的60个个体进行遗传多样性分析。14个ISSR引物共检测到90个位点,其中多态位点65个,多态位点比率0.722 2。Ne i指数统计结果表明,红松人工林的遗传多样性喜鹊沟种群(0.278 9)大于烈士墓种群(0.271 2),Shannon指数统计结果与Ne i指数相同。研究证实草河口林场红松人工林保存了较多的遗传多样性。
With ISSR method, the paper studied genetic diversity of 60 individuals in two populations of Pinus koraiensis plantation in Benxi CaoHekou forest farm. Through the amplification of ISSR with 14 primers, 90 loci in which 65 were polymorphic were detected and percentage of polymorphic loci was 0. 722 2. Nei' s index showed the levels of genetic diversity of Xiquegou population (0. 278 9 ) was higher than Lieshimu population (0.271 2) ; Shannon' s index got the same result with Nei' s. This study confirmed that P. koraiensis plantation in CaoHekou forest farm save the more genetic diversity.