中文摘要：

背景高度多态的 interleukin 在 interleukin-10 (IL-10 ) 的倡导者区域的 10 .G (IL10.G ) microsatellitelocated 基因在 IL-10 基因表示和相互关联上施加积极 transcriptionalregulatory 效果与在里面 vitro IL-10 分泌物。Thisstudy 被进行调查 EL10.G 微卫星是否与发生和 /or 被联系严重败血的结果。在大学医院的特别护理单位被对待的有严重败血的 115 个病人被学习的方法。141 个健康个人用作控制。IL10.G 微卫星 genotyping 与下列二个方法被执行：放大 DNA 染色的荧光灯的基于的聚合酶链反应(PCR ) 技术和银在 polyacrylamide 胶化碎裂。等位基因根据放大 DNA 产品的尺寸被定义。结果十等位基因和 36 遗传型与严重败血并且在健康控制在病人两个都被检测。等位基因 IL10.G9and 等位基因 IL10.G13 在有严重败血的病人分别地是有 32.6% 和 21.3% 的频率的最普通的等位基因，并且 34% 和 27% 分别地在健康控制。IL10.G 微卫星的等位基因频率在有严重败血和健康控制的病人之间也不是不同的(P 】 0.05 ) ，也不在幸存者和非幸存者之间(P 】 0.05 ) 。然而，一普通等位基因 IL10.G13 的频率比在健康控制在有严重败血的病人是稍微更低的(21.3%对27%， P 】 0.05 )，并且等位基因 IL10.G9 的频率比在幸存者在非幸存者是稍微更高的(37.1%对28.1%， P 】 0.05 )。ConclusionIL10.G 微卫星也可以也不不贡献危险性到严重败血到严重败血的致命的结果。

英文摘要：

Background The highly polymorphic intedeukin 10.G （IL10.G） microsatellite located in the promoter region of the interleukin-10 （IL-10） gene exerts a positive transcriptional regulatory effect on IL-10 gene expression and correlates with the in vitro IL-10 secretion. This study was conducted to investigate whether IL10.G microsatellite is associated with the incidence and/or the outcome of severe sepsis. Methods One hundred and fifteen patients with severe sepsis who had been treated at the intensive care unit of the university hospital were studied. One hundred and forty-one healthy individuals served as controls. IL10.G microsatellite genotyping was performed with the following two methods： fluorescent based polymerase chain reaction （PCR） techniques and silver staining of the amplified DNA fragment in polyacrylamide gel. Alleles were defined according to the size of the amplified DNA product. Results Ten alleles and 36 genotypes were detected both in the patients with severe sepsis and in the healthy controls. Allele IL10.G9 and allele IL10.G13 were the commonest alleles with the frequencies of 32.6% and 21.3% respectively in the patients with severe sepsis, and 34% and 27% respectively in the healthy controls. The allele frequencies of IL10.G microsatellite were neither different between the patients with severe sepsis and the healthy controls （P 〉 0.05）, nor between survivors and non-survivors （P 〉 0.05）. However, the frequency of one common allele IL10.G13 was slightly lower in the patients with severe sepsis than in the healthy controls （21.3% vs 27%, P 〉 0.05）, and the frequency of allele IL10.G9 was slightly higher in the non-survivors than in the survivors （37.1% vs 28.1%, P 〉 0.05）. Conclusion IL10.G microsatellite may neither contribute to the susceptibility to severe sepsis nor to the fatal outcome of severe sepsis.