【目的】获取促生同时可防治3种土传真菌病害(Fusarium oxysporum、Sclerotinia sclerotiorum和Rhizoctonia solani)的生防菌,并明确其抑菌效果。【方法】利用前期研究获得的17株促生菌,采用平板对峙法测定其对病原真菌的拮抗作用及对菌丝生长的抑制作用。【结果】可有效拮抗立枯丝核菌的生防菌有6株,其中促生菌株FX2和LM4-3的抑制率达73.82%;拮抗尖孢镰刀菌的生防菌有7株,其中FX2的抑制率达到66.81%;拮抗油菜菌核病菌的生防菌有4株,其中菌株LHS11的抑制率高达85.71%。菌株LHS11和JM170通过次生代谢物抑制病原真菌。所有的生防菌对病原菌的菌丝生长均有一定的抑制作用。【结论】筛选得到对3种真菌病害病原具有较好生防作用的菌株LHS11和FX2。
[Objective] Selecting biocontrol agents for preventing three kinds of soil-borne diseases(Fusarium oxysporum,Sclerotinia sclerotiorum and Rhizoctonia solani),promoting plant growth and clarifying their inhibitory efficiency as well.[Methods] Seventeen plant growth promoting rhizobacteria(PGPR) strains,acquired from preliminary studies,were used to test antagonism on pathogenic fungi by panel confrontation method and to measure the inhibition of mycelial growth by the PGPR strains.[Results] There were six strains efficiently antagonized Rhizoctonia solani.Among the six strains,the inhibition rate FX2 and LM4-3 was higher which could rise up to 73.82%.Seven strains efficiently antagonized Fusarium oxysporum,the inhibition rate of FX2 reached 66.81%.Four strains efficiently antagonized Sclerotinia sclerotiorum,the inhibition rate of strain LHS11 reached 85.71%.The strains of JM170 and LHS11 could prevent disease by secondary metabolites.All strains had a certain impact on mycelial growth of pathogenic fungi.[Conclusion] Strains LHS11 and FX2 that were screened had better biocontrol effects on three soil-borne fungous pathogen.