中文摘要：

目的：观察蛆虫对脊髓损伤后压疮创面细菌感染的抗菌作用。 方法：①以脊髓损伤后压疮创面上分离培养出的金黄色葡萄球菌和绿脓杆菌为指示菌，采用针刺细菌感染诱导丝光绿蝇蛆虫后，提取血淋巴分泌物，平板法做抑菌实验，以未经感染诱导的蛆虫血淋巴分泌物和头孢哌酮盐水溶液做对照，培养24h，记录抑菌圈直径。②选取2005-09／10于大连大学附属医院就诊的5例脊髓损伤后压疮创面细菌感染的成年男性患者，行蛆虫清创治疗，将饥饿状态蛆虫进行虫体消毒后放入压疮创面内，每次放入100条，隔日更换，观察创面情况并做菌培养。 结果：①经细菌感染诱导的蛆虫分泌物对金黄色葡萄球菌和绿脓杆菌的抑菌圈直径大于未感染诱导组和头孢哌酮组[（14．8±1．8），（10．3±0．9），（10．2±1．1）mm；（13．7±1．6），（10．4±0．8），（10．3±0．7）mm，P〈0．05,0．01]。②15例压疮感染病例平均治疗9d，创面坏死组织均清除干净，新鲜的肉芽组织生长，创面培养无细菌生长。 结论：蛆虫对脊髓损伤后压疮创面细菌感染具有较强的抗菌作用。

英文摘要：

AIM： To observe the antibacterial function of maggot therapeutics for decubitus ulcer after spinal cord injury （SCI）. METHODS： （1）Using Staphylococcus aureus and Pseudomonas aeruginosa which were isolated from the infective wound of decubitus ulcer after SCI as the indicators, the hemolymph secretion of the larvae was extracted after induction by the bacterial infection with pricking. Then the antibacterial activity was tested with plate method, and the hemolymph secretions of the larvae without induction of the bacterium and cefoperasone sodium were taken as control groups. The diameter of antibacterial circle was recorded after 24-hour culture. （2）Five adult male patients suffered from decubitus ulcer after SCI were selected from Dalian University Affiliated Hospital between September and October 2005, and treated with maggot debridement therapeutics. The sterilized maggots at hunger were placed in the infective wound of decubitus ulcer, 100 ones once and changed every other day, then the wound surface was observed and bacterium culture was performed. RESULTS： （1）The secretion of the larvae induced by the bacterium had stronger antibacterial activity on Staphylococcus aureus and Pseudomonas aeruginosa, compared with that without induction of bacterium and cefoperazone sodium controls [（14.8 ±1.8）, （10.3 ±0.9）, （10.2 ±1.1） mm; （13.7±1.6）, （10.4±0.8）, （10.3±0.7） mm, P 〈 0.05, 0.01].（2）Five cases of decubitus ulcer infection were cured for 9 days averagely, and fresh granulation tissue developed, necrosis tissue was cleaned. No bacterium existed on the decubitus ulcer surface after bacterium culture. CONCLUSION： Maggot therapeutics has effective antibacterial function on decubitus ulcer after SCI.