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荧光探针法研究牛血清白蛋白和免疫球蛋白的表面疏水特性

ISSN号：1003-9015
期刊名称：高校化学工程学报
时间：2014.8.15
页码：771-776
分类：TQ937[化学工程]
作者机构：[1]浙江大学生物质化工教育部重点实验室,浙江杭州310027, [2]浙江大学化学工程与生物工程学系,浙江杭州310027
相关基金：国家自然科学基金（21036005,21276228）;浙江省自然科学基金（LRl2806003）.
相关项目：抗体分离过程中的介质、方法和集成化研究

关键词：表面疏水性, 荧光探针, 8-苯氨基-1-萘磺酸, 牛血清白蛋白, 牛免疫球蛋白, surface hydrophobicity, fluorescence probe, 8-anilino-l-naphthalene sulfonate, bovineserum albumin, bovine immunoglobulin G

中文摘要：

利用8-苯氨基-1-萘磺酸（ANS）荧光探针法，测定了牛血清白蛋白（BSA）与牛免疫球蛋白（bIgG）的表面疏水性，探讨了pH、温度、添加盐、十二烷基磺酸钠（SDS）、盐酸胍和辛酸钠（NaCA）对蛋白表面疏水性指数So的影响。实验表明，对于阴离子型荧光探针ANS，pH对So测定的影响显著，选择pH略高于蛋白等电点比较合适。温度对两种蛋白So的影响不同，从50℃升至80℃，BSA表面疏水性降低，bIgG表面疏水性升高。添加盐促使蛋白的表面疏水性增强，相比BSA，bIgG增加幅度较大。不同变性剂对两种蛋白So的影响也不同，与变性机制、蛋白疏水特性及分子结构有关。添加NaCA对两种蛋白So影响存在显著差异，随着NaCA浓度升高，BSA的So值减小，而bIgG则维持基本不变，这与疏水层析分离过程的NaCA影响相一致。结果表明，ANS荧光探针法可用于考察不同溶液条件对蛋白质分子表面疏水性的影响，研究特定添加剂一蛋白质间疏水相互作用。

英文摘要：

The surface hydrophobicities of bovine serum albumin （BSA） and bovine immunoglobulin G （bIgG） were studied by a fluorescence method with 8-anilino-l-naphthalene sulfonate （ANS） as the probe. Effects of factors including pH, temperature and additives on the surface hydrophobicity indexes （So） were investigated. The results show that pH can significantly affect the determination of So, as ANS is an anionic fluorescent probe. Solution pH slightly higher than isoelectric point of proteins is suitable. Temperature has different effects on So of BSA and bIgG. So of BSA decreases from 50 ℃ to 80℃, while that of bIgG increases. Salt addition could enhance the surface hydrophobicity of both proteins, and bIgG has much greater variation than that of BSA. Depending on the denaturation mechanisms, surface hydrophobic properties and molecular structure of proteins, the denaturants have different impacts on So of the two proteins. With the increase of sodium caprylate （NaCA） concentration, So of BSA declines and that of bIgG remaines unchanges. This result is consistent with the impacts of NaCA on the separation of BSA and bIgG in hydrophobic interaction chromatography. This study demonstrates that the ANS fluorescence probe method can be used to investigate the effects of liquid conditions on protein surface hydrophobicity and study the hydrophobic interactions between specific additives and proteins.