中文摘要：

雨生红球藻是一种单细胞绿藻，在多种逆境胁迫条件下能够大量合成并迅速积累虾青素，其积累量最高可达细胞干重的4％，从而成为目前最理想的天然虾青素合成工具．八氢番茄红素合成酶（PSY）是虾青素合成途径中第一个限速酶．分离了八氢番茄红素合成酶基因（psy）的全长cDNA及基因组DNA．其全长cDNA包括1200个碱基，编码400个氨基酸，基因组DNA包括5个外显子，4个内含子．系统发育分析结果显示，绿藻的八氢番茄红素合成酶基因形成一个进化枝，它们与高等植物的psy亲缘关系比较近．通过Genome Walking的方法，分离了psy基因约1kb的5’侧翼序列．将含有TATA—box和CAAT—box的297bp的序列与LacZ报告基因构成嵌合的表达载体，用基因枪法转化雨生红球藻．1acZ的瞬间表达检测结果表明，这段上游序列能够驱动lacZ表达，具有启动子活性．

英文摘要：

The unicellular green alga Haematococcus pluvialis accumulates a highly valuable ketocarotenoid, i.e. astaxanthin up to 4% dry weight under stress conditions. Phytoene synthase is considered to be the first rate limiting enzyme in carotenoid biosynthesis pathway in H. pluvial/s. The cDNA and genomic genes of phytoene synthase, i.e. psy fi＇om H.pluvialis were cloned and characterized. Result showed that psy had one open reading flame of 1 200 bp encoding a putative polypeptide of 400 amino acids which was interrupted by four introns. Phylogenetic analysis revealed that psy fi＇om green algae formed a monophyletic clade, and its closer relationship was higher plants. By using genomic walking approach, an approximate 1 kb 5＇ flanking region ofpsy gene was cloned and a number of putative c/s-regulatory elements were revealed. Fusing a 297 bp internal sequence （-297 to -1 bp from the translation initiation codon ofpsy） with the reporter gene, i.e. lacZ before attempted introducing the construct into the green alga via particle bombardment resulted in lacZ transient expression.