中文摘要：

应用紫外-可见光谱法和荧光光谱法并以中性红（NR）作光谱探针研究了Sm（Ⅲ）HP配合物与DNA的作用机制。研究表明，血卟啉（HP）与Sm（i11）的配合比nSm（Ⅲ）：nHP=1：1，Sm（Ⅲ）HP配合物与DNA的结合比nSm（Ⅲ）HP：nDNA=1：1，Sm（Ⅲ）HP配合物与DNA的结合常数K30℃^θ=1．67×10^4L／mol。该过程为熵所驱动，其△r巩。为4．86×10^4J／mol，△rSm^θ为195．61J／（mol·K），30℃时△rGm^θ为-1．06×10^4J／mol。Scatchard法和探针法研究表明，Sm（Ⅲ）HP与DNA之间的结合方式主要是沟区作用。

英文摘要：

Using neutral red （NR） as a probe, functionary mechanism between Sm （ Ⅲ ） HP and DNA was studied by UV-vis and fluorescence spectroscopy. The results indicated that the binding ratio of nsm（Ⅲ） ： nHP is 1 ： 1, nsm（Ⅲ）HP： nDNA =1 ： 1, and the binding constant of Sm （ Ⅲ ） HP complex with Herring Sperm DNA was K30℃^θ = 1.67 × 10^4 L/mol. Thermodynamic parameters of enthalpy （△rHm^θ）, entropy （△rSm^θ） , and Gibbs free energy （△rGm^θ） were calculated to be 4. 86 × 104 J/mol, 195.61 J/ （ mol · K） and - 1.06 × 104 J/mol, respectively. The results suggested that the process of interaction of Sm （ Ⅲ ） HP complex and Herring Sperm DNA was driven by entropy. The results of scatchard and probe methods suggested that the primary interaction mode between Sm （ Ⅲ ） HP and DNA was groove binding.