中文摘要：

【目的】通过开发抗玉米粗缩病实用分子标记,实施标记辅助选择可以明显提高抗病育种效率。【方法】在对国内外152份玉米自交系粗缩病抗性鉴定的基础上,利用抗病自交系和感病自交系基因组DNA构建基因池,结合AFLP技术筛选多态扩增片段;在抗池及抗病自交系与感池及感病自交系间筛选出表现一致的多态片段转化为SCAR标记;通过152份玉米自交系粗缩病抗性鉴定验证标记与玉米粗缩病的相关性。【结果】筛选到2个与玉米粗缩病抗性显著相关的SCAR标记,即SCAR69和SCAR74。【结论】开发的SCAR（SCAR69和SCAR74）标记可应用于抗玉米粗缩病毒分子标记辅助选择。

英文摘要：

【Objective】Developing and cultivating resistant hybrids is an effective approach to control MRDD.Marker development and markers-assisted selection（MAS） is an efficient way for MRDD-resistant breeding.【Method】On the basis of MRDD resistance evaluation of 152 maize inbred lines,resistant and susceptible DNA bulks were composed by using genomic DNAs of 10 resistant and 10 susceptible inbred lines,respectively.Polymorphic AFLP markers were screened between two bulks and then transformed into SCAR（sequence characterized amplified region） markers.Identification of correlation between the SCAR markers and MRDD resistance was carried out in 152 inbred lines.【Result】SCAR69 and SCAR74 were validated to be highly associated with MRDD resistance.【Conclusion】SCAR69 and SCAR74 could be used for MAS of MRDD resistance in maize.