将小麦幼苗放在含有不同浓度磷元素(1 mmol/L和10 mmol/L)和铜元素(0.16μmol/L、100μmol/L和1 mmol/L)的培养液中处理12 h后,观察植物体内铜元素的含量、叶绿素含量、抗坏血酸循环的变化.结果表明,当培养液中施加的铜元素浓度相同时,施加较高浓度的磷(10 mmol/L)能促进植物根部吸收和积累铜,并能促进铜元素从根部向地上部的运输.此外,实验结果还表明,当培养液中都含有较少量的铜(0.16μmol/L)元素时,比较磷浓度对植物体内生理反应的影响时,发现在含有高浓度磷的培养液中培养的小麦体内的受到较少的膜质过氧化损伤,光合色素的含量保持稳定.但是,当培养液中有较高浓度的铜元素(100μmol/L和1 mmol/L)时,施加高浓度的磷反而加剧了植物体内的膜质过氧化损伤和引起光合色素的分解,从而不利于植物正常的生长发育.
Wheat were cultivated in hydroponic culture solution with supplies of copper (0. 16μmol/L, 100μmoL/L and 1 mmol/L) and phosphorus ( 1 mmol/L and 10 mmol/L) under controlled growth conditions. The copper concentrations of leaf and roots, the pigments, ascrobate acid contents and enzyme activities were investigated during 12 h treatment. It was observed that treatments of seedlings with 10 mmol/L phosphate for 12 h caused a remarkable copper accumulation in both leaf and roots. However, the different copper concentrations induced different physiology reaction in plant leaves. Under 0. 16 μmol/L copper treatment condition, 10 mmol/L phosphate decreased oxidative stress, inhibited chlorophyll decomposition, and ascorbate acid cycle had no obvious change regarding to the control plant. Under 100μmol/L and 1 mmol/L copper treatment conditions, 10 mmol/L phosphate increase oxidative stress, made chlorophyll decomposition, and activated ascrobate acid cycle to eliminate reactive oxygen species.