中文摘要：

在获得凋亡蛋白融合基因的基础上，成功地构建了重组表达质粒PET-22b-SPA，将阳性重组质粒转化表达受体菌BL21（DE3）感受态细胞，经IPTG诱导表达，表达产物经聚丙烯酰胺凝胶电泳检测，凋亡蛋白融合基因获得高效表达。软件分析结果表明表达蛋白占菌体蛋白的20％，上清表达量约为10％。上清蛋白经纯化后。Western blot结果显示，利用凋亡蛋白单克隆抗体可以很好地与所表达的蛋白带特异性结合。

英文摘要：

Recombinant expression plasmid pET-22b-SPA was constructed according to recombinant plasmid.The correct recombinant expression plasmid was transformed into the host .strains BL21 （DE3） induced by IPTG. The specific expressed protein was detected by SDS-PAGE . The recombinant protein was expressed at high level accounting for 20 % of the total bacterial protein analyzed by computer software. The amount in supematant is about 10%. After the supernatant protein was purified, western blot showed the monoelonal antibody raised against the recombinant apoptin in murines could react to the protein expressed specifically.