利用分子标记对来自21个国家的745份小麦品种的株高(Rht-B1b和Rht-D1b)、粒重相关基因(TaCwi-A1a和Hap-6A-A)和Lr34/Yr18/Pm38基因进行检测。结果表明:(1)在745份品种中,42.1%和28.7%的材料分别携带Rht-B1b和Rht-D1b等位变异,分布频率在不同国家差异很大。一般来说,来自同一个国家的材料主要携带矮秆基因Rht-B1b或Rht-D1b之一,只有意大利和澳大利亚这两种矮秆基因的频率均较高,而高纬度地区如加拿大和俄罗斯等对株高要求不严,矮秆基因分布频率很低;(2)78.4%的材料携带TaCwi-A1a等位变异,除日本(50.0%)、德国(45.3%)和智利(48.8%)外,其他国家材料中TaCwi-A1a分布频率均很高。29.3%的材料在TaGW2-6A位点携带Hap-6A-A等位变异,主要分布在春性和弱冬性小麦品种中,而冬性和强冬性品种中Hap-6A-G分布较为广泛;(3)22.1%的材料携带Lr34/Yr18/Pm38,美国(18.5%)、乌克兰(28.6%)、俄罗斯(26.1%)、伊朗(20.0%)、土耳其(34.8%)、匈牙利(50.0%)、保加利亚(38.9%)、罗马尼亚(87.0%)、日本(80.0%)、加拿大(34.6%)和澳大利亚(44.6%)分布频率较高;(4)TaCwi-A1的分子标记CWI21和CWI22能很好区分等位变异TaCwi-A1a和TaCwi-A1b,TaGW2-6A的CAPS标记能很好区分Hap-6A-A和Hap-6A-G,准确性高、重复性好,可作为千粒重选择的有效标记。
Using the molecular markers for dwarfing genes(Rht-B1b and Rht-D1b)and kernel weight related genes(TaCwi-A1a and Hap-6A-A) and Lr34/Yr18/Pm38,the distributions of these genes were detected among 745 cultivars from 21 major wheat-growing countries.The results indicated that:(1) The frequencies of Rht-B1b and Rht-D1b in 745 cultivar were 42.1% and 28.7%,respectively,varying much in different wheat-growing countries.Generally,cultivars from the same country usually carried only one of Rht-B1b and Rht-D1b,frequencies only from Italy and Australia were higher for both Rht-B1b and Rht-D1b,which in high latitude areas such as Canada and Russia were lower due to less requirement for plant height.(2) TaCwi-A1a allele was widely distributed in 21 countries with a total frequency of 78.4%,and cultivars from different countries possessed all higher frequency of TaCwi-A1a except for Japan(50.0%),Germany(45.3%) and Chile(48.8%).The 29.3% of cultivars carried Hap-6A-A allele at TaGW2-6A locus,mainly distributing in spring and weak winter wheat,whereas Hap-6A-G was mainly present in winter and strong winter cultivars.(3) The 22.1% of cultivars had Lr34/Yr18/Pm38 allele,with higher frequency in USA(18.5%),Ukraine(28.6%),Russia(26.1%),Iran(20.0%),Turkey(34.8%),Hungary(50.0%),Bulgaria(38.9%),Romania(87.0%),Japan(80.0%),Canada(34.6%) and Australia(44.6%).(4)The molecular markers CWI 21 and CWI 22 for TaCwi-A1 can well differentiate TaCwi-A1a and TaCwi-A1b alleles,while the CAPS marker of TaGW2-6A can also be used for kernel weight selection due to its ability of discriminating Hap-6A-A and Hap-6A-G with great accuracy and repeatability.The information is very crucial for use of exotic germplasm in Chinese wheat breeding program.