中文摘要：

利用对六氯苯（HCB）有了较好降解活性的厌氧混合菌群反应系统,以HCB驯化0 d、60 d、90 d、180 d、240 d和480 d（编号为1~6）的厌氧污泥作为样品（每次取样约为100 mg污泥）,利用PCR-DGGE、DGGE条带测序等方法对降解HCB的厌氧菌群群落结构演变进行了分析。结果表明,对提取的6个样品总DNA进行了PCR扩增,其片段大小约为245 bp;驯化前和驯化60 d的样品,菌种丰富度比较高,而随着驯化时间的延长,样品条带数减少,但部分条带信号明显被强化,说明HCB驯化使污泥中微生物种群和数量得到了定向富集。应用QuantityOne软件分析DGGE图谱不同样品间的相似程度,结果表明,驯化前污泥与驯化不同时期的污泥样品微生物相似性变化呈现降低-增大-再降低的过程,说明HCB驯化过程中微生物群落结构经历了复杂的演替变化。对6号样品的1~4个条带,进行了测序,测序结果表明,降解HCB的优势菌群包含四类菌群,但是目前尚无法单独培养。

英文摘要：

Anaerobic mixed flora acclimated by hexachlorobenzene（HCB） had a better degradation of reactive systems. About 100 mg of anaerobic sludge were sampled which were acclimated by HCB in 0 d, 60 d, 90 d, 180 d, 240 d, and 480 d（named 1~6 sample respectively）. Total DNA of each sample were extracted and evolution of community structure of anaerobic flora were analyzed by PCR-DGGE and sequencing of DGGE bands. The results showed that the fragments length of PCR amplification were approximately 245 bp.Bacterial richness of 0 d and 60 d were relatively higher, with the extension of the acclimatization period, sample bands were declined but specific band signal were obviously intensified, which indicating microbial population and quantity were directionally enriched through HCB acclimation. Microbial similarity degree of DGGE profile of different samples were analysed by QuantityOne software, it showed a tendency of decreasing, increasing, then decreasing of various stage sludge, indicating during HCB acclimation, microbial community structure had undergone complicated succession. 1~4 bands of 6# sample were sequenced and sequencing results showed predominant flora which degraded HCB were consisted of four groups, but not yet cultured alone.