中文摘要：

以雨生红球藻提取物为原料,利用皂化法和溶剂分配法制备虾红素,并研究其结构及稳定性。通过优化皂化过程中的碱液浓度、反应时间和反应温度,得到最佳的工艺条件：碱液浓度7%,皂化时间120min,温度15℃。此条件下,虾红素的转化率达96.12%,制备的虾红素得率为62.16%,纯度达80.36%。用HPLC-DAD-（APCI）MS/MS分析反应产物,解析虾红素的质谱裂解规律,研究光照条件、金属离子和温度对虾红素稳定性的影响。结果表明：紫外光照处理对虾红素稳定性影响显著;Fe^3＋、Ca^2＋、Cu^2＋对虾红素有不同程度的破坏作用,Zn^2＋、Mg^2＋、Na^＋、Fe^2＋对虾红素影响较小;pH在3-8范围内,随着pH升高,虾红素稳定性提高;随着温度的升高,虾红素的保留率下降。本研究为虾红素的活性、毒理及其在食品中的应用等研究提供了重要参考。

英文摘要：

Astacene is a kind of carotenoid which can be used as colorant and antioxidant.Its structure is similar to astaxanthin.While astaxanthin has been studied for years,no much attention has been paid on astacene.The purpose of this study is to prepare astacene from Haematococcus pluvislis extract and to study some properties of it.In this study,Haematococcus pluvislis extract was used as the raw material of preparing purifying astacene with saponification method and solvent distribution method with its structure and stability determined.In terms of the saponification condition,alkali concentration,reaction time and reaction temperature were optimized.The optimal condition was as follows：the concentration of alkali was 7%,saponification time was 120 min,and the temperature was 15 ℃.After saponification under this condition,the saponification solution was placed under 4℃ overnight to make astacene precipitate as much as possible.Then the solution was centrifuged and the sediment was washed with HCl-ethonal solution（v/v,1∶9）and 50% ethonal for 4-5 times to remove alkali and impurities.At last,the sediment was freeze-dried and astacene was obtained.The conversion rate of astacene was up to 96.12% with a 62.16% yield of astacene.The purity of the sample was 80.36%.The product was analyzed by HPLC-DAD-（APCI）MS/MS,and the MS decomposition rule of astacene was parsed.It was found that astacene mainly lost water and ruptured on its conjugated double bonds in MS/MS.Finally,the effects of light conditions,metal ions,pH and temperature on astacene stability were studied.The results showed that ultraviolet had the strongest damaging effect on astacene.Fe^3＋,Ca^2＋ and Cu^2＋caused damage to astacne at different degrees,while Zn^2＋,Mg^2＋,Na^＋ and Fe^2＋ had little effect on it.Acidity between pH 3 and 8,the stability of astacene increased with pH.With the increase of temperature,retention rate of astacene decreased.This study provided a reference for the research of astacene in terms of its activity,toxi