中文摘要：

目的探讨蛋白激酶D1（protein kinaseD1，PKDl）在子宫内膜癌组织和正常子宫内膜组织中的表达，并分析其与子宫内膜癌临床病理特征的关系。方法应用免疫组化sP法及qRT-PCR法检测92例子宫内膜癌组织和48例正常子宫内膜组织中PKDlmRNA及其蛋白的表达，分析PKDl在子宫内膜癌组织中的表达及其与分化程度、临床分期的关系。应用Western blot法检测PKDl蛋白在正常子宫内膜细胞株及不同分化程度的子宫内膜癌细胞株中的表达水平。结果子宫内膜癌组织中的PKDlmRNA及蛋白表达水平均显著高于正常子宫内膜组织（P〈0．01）；PKDlmRNA及蛋白表达与子宫内膜癌的组织分化程度均有相关性，且组织分化程度越低，临床病理分期越高，PKDl的表达越丰富。PKDl蛋白在子宫内膜癌细胞株中的表达水平也远高于正常子宫内膜细胞中的表达（P〈0．01），且癌细胞株的分化水平越低，PKDl蛋白表达的水平越高。结论PKDl在子宫内膜癌患者癌灶中呈高表达，PKDl表达水平的高、低可以做为预测子宫内膜癌恶性程度的一项重要参考依据。

英文摘要：

Purpose To investigate the expression of pro- tein kinase D1 （ PKD1 ） in endometrial carcinoma and normal endometrium, and to investigate its relationship with the clinieo- pathological features of endometrial carcinoma. Methods Im- munohistochemical SP and qRT-PCR was used to detect the mR- NA and protein expression of PKD1 in 92 eases of endometrial cancer and 48 cases of normal endometrium, and to analyze the relationship of expression of PKD1 with the tumor differentiation and clinical stage of endometrial carcinoma tissue. Western blot method was used to detect the expression level of PKD1 protein in normal endometrial cell line and endometrial carcinoma cell lines with different degree of differentiation. Results mRNA and protein expression of PKD1 in endometrial carcinoma tissueswas significantly higher than those in normal endometrial tissues （P 〈0. 01 ） , which showing a correlation to the degree of tissue differentiation and clinical pathologic staging. While, the ex- pression level of PKD1 protein in endometrial cancer cell lines was also much higher than that in normal endometrial cells （ P 〈 0.01 ） , and the lower differentiation, the higher level of PKD1 protein expression. Conclusion PKD1 is highly expressed in endometrial cancer patients. The level of PKD1 expression may be an important reference for predicting the malignant degree of endometrial cancer.