通过转录组测序获得了金荞麦F3'5'H基因cDNA序列,生物信息学分析表明,金荞麦F3'5'H基因的cDNA全长为1 871 bp,其中编码区全长1 599 bp,编码532个氨基酸,由进化树分析得知,金荞麦F3'5'H基因属于细胞色素P450 78 A 10家族.
The cDNA sequence of F 3' 5' H gene from Fagopyrum cymosum is obtained using transcriptome sequencing,the cDNA fulllength of F 3' 5' H gene is 187 1 bp,and the fulllength of coding region is 1 599 bp,which encoded 532 amino acids by bioinformatics analysis.And F 3' 5' H gene from buckwheat belongs to the family of cytochrome P 450 78 A 10 after phylogenetic analysis.