中文摘要：

目的 研究乙型肝炎病毒（HBV）在人近端肾小管上皮细胞（HK-2）的表达及对其转分化的影响。方法 体外培养HK-2细胞，分为HK-2组、HK-2-PHY106组（空质粒PHY106转染组）、HK-2-PHY106-HBV组（PHY106-HBV质粒转染组）。用脂质体lipofectamineTM2000转染HK2细胞。用ELISA法检测各组细胞培养上清液中HBsAg与HBeAg的含量。免疫细胞化学染色及Western blot印迹检测转染72h后E-钙黏素（E-cadherin）、α-平滑肌肌动蛋白（α-SMA）的表达。RT-PCR法检测转染72h后转化生长因子-1（TGF-β1）的mRNA。结果 HK-2-PHY106-HBV组细胞培养上清液中可检测到HBsAg和HBeAg的高表达；免疫细胞化学染色及Western blot印迹检测均显示HK-2-PHY106-HBV组E-cadherin表达显著下调（P＜0.05），而α-SMA表达与其他两组相比明显上调（P＜0.05）；RT-PCR显示HK-2-PHY106-HBV组TGF-β1的mRNA表达上调（P＜0.05）。结论 HBV可在HK-2细胞内高效复制，并能够导致HK-2转分化，其机制可能是通过上调TGF-β1来实现的。

英文摘要：

Objective To investigate the expression of HBV and effects on the transdifferentiation in HK-2 cells. Methods HK-2 cells were cultured in vitro and we devided them into the HK-2 group, the HK-2-PHY106-HBV group （HK-2 cells transfected with PHY106-HBV plasmid） and the HK-2-PHY106 group （HK-2 cells transfected with PHY106 control plasmid）. HK-2 cells were transfected by lipofectamineTM2000. Supernatant of culture was collected for examining HBsAg and HBeAg by using ELISA; The expression of E-cadherin and α-smooth muscle actin in HK-2 were assayed by immunocytochemistry and Western blot after the cells were transfected for 72h. TGF-β1 mRNA was detected by RT-PCR after the cells were transfected for 72h. Results The HBsAg and HBeAg were detected with a higher expression in the HK-2-PHY106-HBV group. The E-cadherin detected by immunocytochemistry and Western blot showed a significantly lower expression in the HK-2-PHY106-HBV group（P〈0.05）. However, the expression of α-SMA in the HK-2-PHY106-HBV group was higher than that of the other two groups（P〈0.05）. Additionally, we also found TGF-β1 mRNA expression was upregulated in the HK-2-PHY106-HBV group showed by RT-PCR（P〈0.05）. Conclusion HBV can be replicated highly efficiently in HK-2 cells and can make HK-2 cells occur transdifferentiation. The mechanism may be by upregulating TGF-β1 expression.