中文摘要：

作为组织工程支架材料,脱细胞真皮基质（ADM）孔径小、孔隙率不高、孔隙连通情况不理想,不符合细胞生长对支架材料微结构的理想要求。针对这一问题,选用猪皮为原料,以优化微结构为目的,综合碱处理、酶处理、去污剂处理和盐处理手段制备了一种ADM。扫描电子显微镜分析了碱处理中碱液浓度对ADM微结构的调控作用,MTT法检测了小鼠胚胎成纤维细胞（3T3）在ADM上的生长增殖,共聚焦激光扫描电镜观察了细胞的分布。结果显示：ADM不含细胞,维持了胶原纤维的天然结构,具有连通的三维孔道,孔径约100μm;随着碱液浓度的增加,孔径与孔隙率增大,孔隙的连通性改善,骨架的形貌由膜状向纤维状转变;细胞在ADM上增殖良好、分布均匀。调节碱处理中碱液浓度可调控ADM微结构,获得微结构和细胞相容性良好的ADM。

英文摘要：

Acellular dermal matrix（ADM）has been widely used as a dermal scaffold material in tissue engineering,while its porosity is low and pore interconnectivity is poor.The microstructure is not ideal enough for cell attachment and viability.In light of this,a porcine ADM was prepared by combined treatments with enzyme,alkali,sodium dodecyl sulfonate and sodium chloride solution for forming a desired microstructure.The relationship between the microstructure of the ADM and treatments with various alkali solutions was analyzed by using scanning electron microscopy（SEM）.Mouse embryonic fibroblasts（3T3）were cultured on the ADM and cell proliferation tests were performed by colorimetric MTT assay.The ADM stained with Rhodamine 110 and cells stained with acridine orange were viewed under a confocal laser scanning microscope（CLSM）.SEM images displayed that the ADM made up of natural collagen had three-dimensional interconnected channels with diameter of about 100 μm and no cell was observed.The alkali concentrations affect the pore structure and morphology of the ADM.The MTT assay indicates that the cells cultured on the ADM proliferated well and CLSM image showed even distribution of cells.The prepared ADM had good microstructure and cytocompatibility.It is expected to have potential application in tissue engineering.