中文摘要：

目的利用杂交瘤技术制备分泌抗重组旋毛虫副肌球蛋白N端抗原（rTsP3）的单克隆抗体（McAb）并进行鉴定。方法以rTsP3免疫BALB/c小鼠,取其脾细胞与小鼠骨髓瘤SP2/0细胞融合,筛选分泌高滴度McAb杂交瘤细胞株,制备腹水并进行纯化,采用间接ELISA法测定培养细胞上清液及腹水中的McAb滴度、相对亲和力及抗体亚类,Western blot法鉴定抗体对抗原识别的特异性。结果获得了2株稳定分泌抗旋毛虫rTsP3的McAb杂交瘤细胞株,分泌的McAb分别为IgG2b亚类κ型和IgG1亚类κ型,亲和力常数分别为8.98×108mol/L和9.7×10^8mol/L,Western blot显示2株单抗均能识别旋毛虫成虫匀浆蛋白、rTsP3及重组副肌球蛋白（rTsPmy）。结论成功制备了抗旋毛虫rTsP3单克隆抗体,该单抗能识别旋毛虫副肌球蛋白抗原。

英文摘要：

Objective This study sought to obtain monoclonal antibodies （McAbs） against recombinant protein of the N-terminal end of paramyosin （rTsP3） from Trichinella spiralis. Methods BALB/c mice were immunized with rTsP3. Spleen cells of immunized mice were fused with myeloma cells SP2/0 and hybridoma cell strains secreting high-ti- ter McAbs were selected. Then, the ascites were prepared and purified. The titer of culture supernatants and ascites and sub-classes and affinity of MeAhs were detected by indirect ELISA. The specificity of the McAbs was also examined by Western blotting. Results Two hybridoma cell lines that consistently secreted anti-rTsP3 McAbs have been estab lished. The isotypes of McAbs were determined to be IgG2bk and IgGlk, and their respective affinity constants were 8.98 ×10^8 mol/L, 9. 7 ×10^8 mol/L. Western blotting revealed crude somatic extracts from T. spiralis adult worms, and rTsP3 and recombinant paramyosin （rTsPmy） were specifically recognized by McAbs. Conclusion Two McAbs against rTsP3 that recognized paramyosin of T. spiralis were obtained.