中文摘要：

自我障碍(SI ) 是一个重要交配系统阻止使近交并且支持 outcrossing。ARC1 和 Exo70A1 在 Brassica SI 发信号作为 S 地点受体 kinase 和戏保守人士角色的下游的目标工作。基于顺序相同， Exo70A1 被划分成四子域：白氨酸拉链(列伊 ¹²⁸-Leu¹⁴⁹), hypervariable 区域(重量的单位 ¹⁷²-Leu¹⁹⁷), 相扑修正主题(Glu ²⁶⁰-Ile²⁷⁵), 和 pfamExo70 领域(他的 ²⁷¹-Phe⁶²⁷) 。ARC1 如下包含四个领域：白氨酸拉链(列伊 ¹¹⁶-Leu¹³⁷), 卷卷领域(Thr ²¹⁰-Val²³⁶),U 盒子(毒蛇 ²⁸²-Trp³⁴⁷) 主题，和手臂(翼 ⁴¹⁵-Thr⁶¹¹) 领域。生物信息学分析，酵母二混血儿的屏蔽和 ARC1 _116-236 的白氨酸拉链和卷卷主题为和 Exo70A1 的相互作用被要求的下拉试金表演，当手臂主题的增加导致和 Exo70A1 的相互作用的损失时。同时，没有任何域的 Exo70A1 的N终端与 ARC1 显示出一个弱相互作用，并且 LacZ 表示的水平与白氨酸拉链的增加增加并且与 hypervariable 区域和相扑修正主题到达最大的值，显示那个 hypervariable 区域和 Exo70A1 _172-275的相扑修正主题为有 ARC1 的绑定主要负责，而 pfamExo70 领域为 ARC1 有很少亲密关系。位于 Exo70A1 hypervariable 区域的 Lys ¹⁸¹ 可以是调停的 ubiquitination 地点在 ARC1 和 Exo70A1 之间的相互作用。因此，两有 ARC1 _116-236, 和 hypervariable 区域和 Exo70A1 _172-275 的相扑修正主题的卷卷结构的白氨酸拉链是在 ARC1 和 Exo70A1 之间的核心相互作用领域。影响这些核心领域的任何因素将是调停的 ARC1 的管理者在自我不兼容的系统的 ubiquitin 降级。

英文摘要：

Self-incompatibility （Sl） is an important mating system to prevent inbreeding and promote out- crossing. ARC1 and Exo70A1 function as the downstream targets of the S-locus receptor kinase and play conservative roles in Brassica SI signaling. Based on the sequence homology, Exo70A1 is divided into four subdomains： leucine zipper （Leu128-Leu149）, hypervariable region （Ser172- Leu197）, SUMO modification motif （Glu260-lle275）, and pfamEx070 domain （His271-Phe627）. ARC1 contains four domains as follows： leucine zipper （Leu116-Leu137）, coiled-coil domain （Thr210-Val236）, U-box （Asp282-Trp347） motif, and ARM （Ala415-Thr611） domain. Bioinformatics analysis, yeast two- hybrid screening and pull-down assays show that leucine zipper and coiled-coil motifs of ARCl116-236 are required for the interaction with Exo70A1, while the addition of ARM motif results in loss of the interaction with Exo70Al. Meanwhile, the N-terminal of Exo70A1 without any domains shows a weak interaction with ARC1, and the level of LacZ expression increases with addition of leucine zipper and reaches the maximum value with hypervariable region and SUMO modification motif, indicating that hypervariable region and SUMO modification motif of Exo70A1172-275 is mainly responsible for the binding with ARC1, whereas pfamEx070 domain has little affinity for ARC1. Lys181 located in the Exo70A1 hypervariable region may be the ubiquitination site mediating the interaction between ARC1 and Exo70A1. Therefore, both the leucine zipper with coiled-coil struc- ture of ARC1116-236, and the hypervariable region and SUMO modification motif of Exo70A1172-275 are the core interaction domains between ARC1 and Exo70A1. Any factors affecting these core domains would be the regulators of ARC1 mediating ubiquitin degradation in self-incompatible system.