中文摘要：

为探讨锌指蛋白在大豆对非生物逆境胁迫耐受过程中的作用,构建了锌指蛋白基因SCTF-1的植物表达载体p CPB-SCTF-1,利用花粉管通道法将其导入大豆中,通过抗性筛选及PCR检测,共获得了6株转SCTF-1基因植株,Southern杂交鉴定表明功能元件以单拷贝形式整合于受体基因组中。荧光定量PCR检测证明转化植株在根、茎、叶部位的表达与未转化植株相比显著提高。在4℃低温胁迫下,转SCTF-1基因植株相对电导率明显低于非转化植株,降低了21.10%～23.09%;丙二醛含量明显低于非转化植株,降低了10.56%～11.74%;过氧化物酶活性明显高于非转化植株,增加了25.02%～30.38%;转基因植株叶片未呈现明显的萎缩、萎蔫、打卷现象。因此,转SCTF-1基因的过量表达提高了转基因大豆的耐冷能力。

英文摘要：

The zinc finger protein in plant plays an important role in resistance to abiotic stresses. In this research,a cold resistance gene,SCTF- 1,was introduced into soybeans via pollen tube pathway transformation. Six positive transgenic plants were obtained by PCR detection. Southern blotting showed that SCTF- 1 was integrated into the genome of recipient soybean as a single copy. The quantitative real- time PCR showed that the expression of introduced SCTF- 1 gene increased significantly in root,stem and leaf compared to the non- transformed soybean. Under the stress of low temperature,compared to non- transformed plants,the relative electrical conductivity of transformed plants were significantly lower with 21. 10% to 23. 09% lower value; the content of malondialdehyde（ MDA） of transformed plants reduced 10. 56% to 11. 74%,the peroxidase activity of transformed plants increased25. 02% to 30. 38%. There is no atrophy,wilting and rolling in the leaves of transgenic plants. Therefore,the cold tolerance of transformed plants was significantly enhanced by overexpressing of SCTF- 1 gene.