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沙打旺根腐病菌的生物学特性研究
  • 期刊名称:内蒙古农业大学学报(自然科学版)
  • 时间:0
  • 页码:132-137
  • 语言:中文
  • 分类:S541.901[农业科学—作物学]
  • 作者机构:[1]内蒙古农业大学农学院,呼和浩特010019, [2]兴安盟农业局农业技术推广站,乌兰浩特137400, [3]中国农业大学农业生物与技术学院,北京100193
  • 相关基金:基金项目:国家自然科学基金项目(30860166);国家“863”计划现代农业技术领域重大项目(2006AA10A211)
  • 相关项目:荧光假单胞菌抗生素2,4-二乙酰基间苯三酚合成的转录调控
中文摘要:

对引起沙打旺根腐病的2种病原菌茄病镰刀菌沙打旺专化型(Fusarium solani f.sp astrgali,Sad1)和尖孢镰刀菌沙打旺专化型(Fusarium oxysporum f.spastrgali,Sad2)的生物学特性进行了研究。结果表明Sad1和Sad2均能在PDA、燕麦、沙打旺汁液、czapek等固体和液体培养基上生长,PDA和燕麦固体培养基是最适条件 但液体培养基以czapek为最适条件。Sad1和Sad2最适液体产孢条件是燕麦培养基,最适固体产孢条件是PDA。Sad1在5℃~40℃均能生长,适温是25℃~30℃,在5℃时生长缓慢 pH3~12范围内都能生长,25℃适温条件下pH6.0~8.0 Sad2在5℃~35℃均能生长,适温是25℃~30℃ pH3~12范围内都能生长,25℃适温条件pH9.0~11.0 2菌均能利用葡萄糖、蔗糖、果糖、麦芽糖、甘油和可溶性淀粉等多种碳源 也能利用天门冬酰胺、干酪素、谷氨酸,但对蛋白胨利用较差,不利用柠檬酸和酒石酸。生物学特性表明,2菌株是一类对营养要求不高,对环境适应能力强的病原菌。

英文摘要:

Biological characters of two kinds of pathogens ( Fusarium solani ~ sp astrgali, Sadl and FusaHum oxysporum f. sp astrga- li, Sad2) causing Sha Da Wang Root - Rot disease were studied. The results show that both Sadl and Sad2 can grow on solid or liquid medium such as PDA, oat, sap of Sha Da Wang, czapek and so on. The optimal solid media are PDA and oat while the best liquid me- dium is czapek. For the sporulation, oat was the optimal liquid medium, PDA for the solid medium. Sadl can grow in the temperature range of5℃ -40℃, the optimal range is 25℃ -30℃ ; Sad2 can grow within temperature 5℃ ~35℃, the optimal temperature range fell at 25℃ -30℃ ; pH range for growth of both is 3 - 12. They both can utilize many kinds of carbon source such as glucose, su- crose, fructose, maltose, glycerol and starch but not citric acid and tartaric acid. Biological characters showed both pathogenic fungi just require relatively poor nutrition for growth and show relatively strong adaption to environment.

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