中文摘要：

目的了解慢性乙型肝炎（cHB）患者外周血单个核细胞来源的树突状细胞Toll样受体3（TLR3）触发后I型IFN表达情况。方法选取CHB患者26例，健康对照者18例，抽取外周血用免疫磁珠细胞分选法获得纯化的单核细胞（同时留取血浆），体外培养诱导为未成熟的树突状细胞，给予聚肌胞苷酸（PolyI：C）刺激，在刺激0、24h收集细胞培养上清液，用ELISA法测定血浆及细胞培养上清液中Ⅰ型IFN（IFN—α、IFN—β）表达水平。均数间两两比较采用t检验。结果在血浆中，两组Ⅰ型IFN表达水平的差异无统计学意义。刺激前（0h），CHB组单个核细胞来源的树突状细胞表达的IFN—α、IFN-β分别为（80．00±16．15）ng／L和（36．39±13．90）ng／L，对照组分别为（76．76±15．90）ng／L和（37．14±13．68）ng／L，差异均无统计学意义（t=1．651，t=0．178；均P〉0．05）。两组刺激后24h的IFN—α表达水平均比刺激前（0h）升高，但差异无统计学意义（t=1．534，t=1．243；均P〉0．05）。刺激后24h，对照组IFN—β水平为（54．57士16．80）ng／L，显著高于刺激前（Oh）的（37．14±13．68）ng／L（t=4．061，P〈0．05）；但在CHB组，两者的差异无统计学意义（t=1．796，P〉0．05）。刺激后24h，两组的IFN-α表达水平差异无统计学意义（t=0．792，P〉0．05），而IFN-β水平在对照组为（54．57±16．80）ng／L，显著高于CHB组的（41．64±12．57）ng／L（t=2．921，P％0．05）。结论CHB患者单个核细胞来源的树突状细胞存在功能障碍，不能正常表达I型IFN TLR3触发后单个核细胞来源的树突状细胞表达的I型IFN以IFN-β为主。

英文摘要：

Objective To detect the expression of type I interferon in monocyte-derived dendritic cells （MoDCs） after Toll like receptor （TLR） 3 triggered in patients with chronic hepatitis B （CHB）, and to evaluate immune responses of CHB patients and its roles in the mechanisms of persistent infection of hepatitis B virus （HBV） and chronicity of hepatitis. Methods Peripheral blood mononuclear ceils （PBMCs） were isolated and purified using magnetic beads （plasma was saved simultaneously） from 26 CHB patients and 18 healthy volunteers （HV）. Dendritic ceils （DCs） were induced and proliferated in a culture medium with recombinant human granulocyte macrophage colony stimulating factor （rhGM-CSF） and recombinant human interleukin （rhlL-4）. DCs were stimulated with Poly I： C and the supernatants were collected at 0 h and 24 h after stimulation. Type I interferon （IFN-α and IFN-β） in plasma and supernatants were examined by enzyme linked immunosorbent assay （ELISA）. Results The levels of type I interferon in plasma were not significantly different in groups of HV and CHB. IFN-α and IFN-β expressions in supernatants before Poly I ： C stimulation were （80.00±16.15） ng/L, （36.39±13.90） ng/Lin CHBgroup and （76. 76±15. 90） ng/L, （37.14±13.68） ng/L in HV group, respectively. And there were no statistical differences between two groups （t= 1. 651, t=0. 178 both P〉0.05）. IFN-α expressions in supernatants at 24 h after stimulation in two groups were both higher than those before stimulation （at 0 h）, but there were no statistical differences （t= 1. 534, t = 1. 243; both P〉0.05）. IFN-β expressions in s upernatants at 24 h after stimulation in HV group was （54.57±16.80） ng/L, which was significantly higher than that at 0 h （37.14±13.68） ng/L （t=4. 061, P〈0.05）. However, there was no significant difference at 24 h than that at 0 h in CHB group （t=l. 796, P〉0.05）. At 24 h after stimulation, IFN-β level was （54.57-＋ 16.80） n