中文摘要：

目的：探讨铁-氟尿嘧啶配合物的抗肿瘤活性和对人胃癌细胞凋亡的影响。方法：采用MTT法检测配合物及其配体对K562、HCT-116、SGC-7901、MCF-7和HEPG-2细胞的增殖抑制作用；Hoechst33342/PI双染法和流式细胞术检测对人胃癌细胞凋亡的影响；RT-qPCR检测对Caspase-3、Bax、Bcl-2基因表达的影响。结果：配合物作用5株细胞后的IC50值分别为7.8×10-5、5.4×10-5、3.5×10-5、1.1×10-4和2.2×10-5mol/L，能明显抑制细胞的增殖。配合物以10-5mol/L浓度作用SGC-7901细胞36h后，凋亡率为9.8%，能明显促进SGC-7901细胞凋亡（P＜0.01），作用强于其配体5-Fu和Phen（P＜0.01）；使凋亡相关基因Caspase-3表达上调至4.9（P＜0.01）；Bcl-2表达下调至0.2（P＜0.01）。结论：铁-氟尿嘧啶配合物具有良好的体外抗肿瘤活性，其发挥抗肿瘤作用可能与诱导肿瘤细胞凋亡有关，而其诱导凋亡的作用可能与Caspase-3基因上调和Bcl-2基因下调有关。

英文摘要：

Objective: To explore iron- fluorouracil complex's anti- tumor activity and effect to human gastric carcinoma cells apoptosis. Methods: MTT method was used to detect inhibition rate of the complex on tumor cell lines: K562, HCT-116, SGC-7901,MCF-7 and HEPG-2; Hoechst 33342/PI and flow cytometry(FCM)methods were used to exhibit apoptosis of SGC-7901 cells; RTqPCRwas used to measure the mRNA expression of apoptosis related factors: Caspase-3, Bax and Bcl-2. Results: Iron-fluorouracil complex obviously inhibited the proliferation of 5 tumor cell lines. IC50 were 7.8×10-5, 5.4×10-5, 3.5×10-5, 1.1×10-4 and 2.2×10-5 mol/L,respectively. After 36 hours' treatment on SGC-7901 cell with a concentration 10- 5 mol/L, its apoptosis rate was 9.8%, which could promote SGC-7901 cell apoptosis than its ligands 5-Fu and Phen(P<0.01); the mRNA expression of Caspase-3 gene was up-regulated to 4.9(P<0.01), while Bcl-2 was down-regulated to 0.2(P<0.01). Conclusion: The complex showed good anti-tumor activity in vitro, up regulation of Caspase-3 as well as down regulation Bcl-2 may be one of its mechanisms of action.