中文摘要：

为探明安徽省某养鹅场60日龄鹅出现的软脚,排白色粪便,严重者导致鹅死亡的原因,对病死鹅进行剖检,发现其以肝脏肿大,脾脏肿大和坏死以及肺脏充血、出血为主要特征。对分离毒株进行RT-PCR扩增试验、序列分析及动物回归试验,结果表明,该分离毒株为鹅呼肠孤病毒（Goose reovirus,GRV）,命名为GRV-LA16株,其ELD50为10-5.7/0.2 m L。病理组织切片结果显示,与对照组相比,肺泡壁胀破融合,毛细血管扩张出血,且肺泡腔有红色丝网状纤维素渗出物。σC基因测序结果分析表明,其与鸭呼肠孤病毒σC基因同源性为87%。GRV-LA16与鸭呼肠孤病毒亲缘关系较近,为同一拓扑群,而与经典的禽呼吸肠孤病毒（Avian orthoreoviruses,ARV）、GRV、番鸭呼肠孤病毒（Muscovy duck reovirus,MDRV）处于不同拓扑群。

英文摘要：

The aim of this study was to isolate and characterize a new Goose reovirus in Anhui province. The diseased geese were weak in legs and diarrhea. Postmortem findings included swollen livers and gray necrotic spots in spleens, hyperaemia and hemorrhage in lungs. By sequencing the specific PCR product of the virus was characterized as a goose reovirus designated as GRV-LA16 strain. ELD50 was titrated to be 1057/0.2 mL. Histopathological examination also found that alveolar walls showed burst fusion. Moreover, the capillaries were dilated and congested, and red fibrinous exudate was in the alveolar spaces. Sequencing of ℃ gene showed that the GRV-LA16 and novel duck reovirus had a close genetic relationship with 87% homology. Phylogenetic analysis of σC gene revealed that GRV-LA16 was not close to Avian orthoreoviruses, GRV and Muscovy duck reovirus.