中文摘要：

为探究荨麻对子宫运动的影响及其可能机制,本试验用生物信号处理系统测定了浓度递增（0.5%、1.0%、2.0%、4.0%、8.0%和16.0%）的麻叶荨麻（Urtica cannabina,UC）醇提液对未孕大鼠离体子宫收缩频率、收缩张力和收缩幅度的影响,并用特异性M-受体阻断剂硫酸阿托品（atropine,A,2.5μg/mL）和2.0%UC联合用药,分为3个处理：UC单独处理、UC＋A和A＋UC,观察UC对子宫收缩的影响是否与M-受体有关。结果显示,在UC浓度递增到4.0%时收缩频率较用药前显著增加（P〈0.05）;浓度递增到4.0%、8.0%时,收缩幅度较用药前极显著降低（P〈0.01）;但浓度递增UC对子宫收缩张力影响不显著（P〉0.05）。一次性加入2.0%UC后子宫收缩张力迅速增加并接近僵直,之后逐渐降低。无论UC＋A或A＋UC处理都使子宫收缩频率较用药前明显加快,收缩张力极显著增加（P〈0.01）,但2个联合用药处理组之间差异不显著（P〉0.05）;UC＋A和A＋UC处理组收缩张力较UC单独用药组均极显著降低（P〈0.01）。结果表明,UC可兴奋未孕大鼠离体子宫,A可缓解UC引起的子宫僵直或张力增加,但不能阻断UC引起的子宫收缩频率增加。提示UC对未孕大鼠离体子宫的兴奋作用和激动与M-受体有一定关系,但可能还存在其他机制。

英文摘要：

To explore the effects of Urtica cannabina（UC）on uterus motility and its possible mechanism,alcohol extracts of UC at increasing doses（0.5%,1.0%,2.0%,4.0%,8.0% and16.0%）were added to non-pregnant rat uterus in vitro.Changes of contraction frequency,contraction tension and contraction amplitude were recorded by biological signal processing system.Moreover,the combined effects of specific M-receptor antagonist atropine sulfate（A）at the dose of 2.5μg/mL and UC at a dose of 2.0% on the motility of rat uterus were tested.There were three treatments,UC alone,UC＋A and A＋UC.The results showed that the contraction frequency was significantly increased when the concentration of UC arrived 4.0%compared with that before treatment（P0.05）;When the concentrations arrived 4.0% and 8.0%,contraction amplitudes of the uterus were extremely significantly decreased compared with that before treatment（P0.01）;However,the contraction tension was not affected by the increasing concentrations of UC（P0.05）.When 2.0% UC alone treatment,uterus contraction tension increased rapidly and was close to rigidity,and then gradually decreased.Contraction frequencies after UC＋A or A＋UC treatments were extremely significantly faster than that before treatment（P0.01）,but the difference between the two combined treatments was not significant（P0.05）.Contraction ten-sion of uterus after UC＋A and A＋UC treatments were extremely significantly reduced compared with that after UC alone treatment（P0.01）.The results indicated that UC could stimulate the contraction of rat uterus partly medicated by M-receptor,whereas there maybe were some other mechanisms involved in the effect.