中文摘要：

目的：探讨原癌基因c—erbB2、c—myb在介导表皮生长因子、肿瘤坏死因子α、内皮素-1及一氧化氮影响卵母细胞成熟中的作用。方法：建立小鼠卵母细胞体外培养模型，观察EGF、TNFα、ET-1及NO供体小剂量硝普钠（SNP）对卵细胞成熟的影响，用RT-PCR和Western blotting方法检测在上述细胞因子作用下卵母细胞c—erbB2和c—myb表达及丝裂原蛋白激酶（MAPK）和成熟促进因子（MPF）含量的变化。结果：EGF（10μg/L）能促进卵母细胞的成熟，使c—erhB2 mRNA、c—myb mRNA表达上升，伴随MAPK磷酸化水平和cyclinB1含量升高；TNFα（10μg／L）和ET-1（100mol／L）均使卵母细胞中c-erbB2 mRNA和c—myb mRNA表达下降，伴随MAPK磷酸化水平和cyclinB1含量下降，抑制卵母细胞的成熟。小剂量SNP（10^-5mol／L）对卵母细胞成熟无明显影响，但能反转二丁酰环磷酸腺苷（dbcAMP）对卵母细胞成熟的抑制作用，使c—erbB2 mRNA和c—mybmRNA表达升高，MAPK磷酸化水平及cyclinB1含量升高，但仍低于对照组：结论：细胞因子对卵母细胞成熟的影响与原癌基因c—erbB2和c—myb有关，c—erbB2和c—myb可能是介导细胞因子和MAPK、MPF间调控卵母细胞成熟的上游激活物。

英文摘要：

Aim： The mechanisms of cytokines in regulating oocyte maturation is still little known. The present study attempt to investigate whether the protooncogene of c-erbB2, c-myb are invoked in introducing of cytokines to regulate oocyte maturation. Methods： This research used mouse GV stage oocyte culture model in vitro and RT-PCR, Western blotting method to explore the effect of EGF, TNFα, ET-1 and NO on oocyte maturation; to analyze the c-erbB2 mRNA and c-myb mRNA expression and the phosphorylation of MAPK and cyclinB1 expression in oocytes affected by above cytokines. Results： EGF（10 μg/L） stimulated meiosis of oocytes significantly, the level of c-erbB2 mRNA, c-myb mRNA were increased, and promoted the phosphorylation of MAPK and cyclinB1 expression; TNFα（ 1 μg/L） and ET-1 （（ 10^-1 mol/L） had the contrary results to EGF. Low dose of SNP（（10^-5mol/L） had no effect on oocyte maturation, but could significantly reverse the suppression of dbcAMP on oocyte maturation. Conclusion： c-erbB2 and c-myb were involved in introducing of cytokines to regulate oocyte maturation, might be the middle link in connection of the cytokines with MAPK and MPF in regulation oocyte maturation.