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小麦品种对增强UV—B辐射响应的RAPD分析
  • ISSN号:1672-2043
  • 期刊名称:《农业环境科学学报》
  • 时间:0
  • 分类:S332.1[农业科学—作物遗传育种;农业科学—农艺学]
  • 作者机构:[1]云南农业大学农学与生物技术学院,云南昆明650201, [2]云南农业大学生态环境研究所,云南昆明650201
  • 相关基金:国家自然科学基金项目(30160023)
中文摘要:

在前一研究结果的基础上,选用8个对UV—B辐射耐受性不同的小麦品种(4个耐性品种和4个敏感品种),进一步对其进行RAPD分析。结果表明,8个小麦品种间存在着明显的遗传多态性。聚类分析显示,在遗传距离为0.35的水平上,可将它们明显区分出耐性和敏感两大类,这与其生长响应指数(RI)的判定结果基本一致。4个耐性品种共同具有1500bp(OPA-12)的特征谱带,这一分子标记可能与小麦耐UV—B辐射相关,有待进一步研究。

英文摘要:

The decline in stratospheric ozone concentration has been confirmed by satellite measurements. Mathematical models predict a further increase in solar UV-B irradiation in further years. Effects of UV-B on plants have been the subjects of considerable research. An examination of more than 200 plant species reveals that roughly 20% are sensitive, 50% are mildly sensitive or tolerant and 30% arc completely insensitive to UV-B radiation. In the past time, we reported that there were intraspecific differences in growth and grain yield response of 10 wheat cuhivars to UV-B radiation, according to response index (RI), 4 sensitive cuhivars and 4 tolerant cuhivars have been determined. Plant species and even genotypes within species can differ greatly in their responses to UV-B. Based on the preceding research results, 8 wheat cuhivars that responded differently to enhanced UV-B radiation, including 4 tolerant cuhivars and 4 sensitive cuhivars, were further made RAPD analysis. The results showed that there was obvious genetic polymorphism among 8 wheat cuhivars. They were divided into two types at the level of 0.35 of genetic distance, that is, 4 cuhivars were tolerant and other 4 were sensitive to enhanced UV-B radiation. This classification was consistent with the result of RI. As a common DNA band of 4 tolerant cuhivars, 1 500bp (OPA-12) was correlated with UV-B-resistibility of wheat, which needed further study.

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期刊信息
  • 《农业环境科学学报》
  • 北大核心期刊(2011版)
  • 主管单位:中华人民共和国农业部
  • 主办单位:农业部环境保护科研监测所 中国农业生态环境保护协会
  • 主编:李文华
  • 地址:天津市南开区复康路31号
  • 邮编:300191
  • 邮箱:caep@vip.163.com
  • 电话:022-23674336 23006209
  • 国际标准刊号:ISSN:1672-2043
  • 国内统一刊号:ISSN:12-1347/S
  • 邮发代号:6-64
  • 获奖情况:
  • 全国中文核心期刊,列于被引频比最高的中国科技期刊500名之内
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,美国剑桥科学文摘,中国中国科技核心期刊,中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:34877