中文摘要：

背景与目的：前列腺亮氨酸拉链（prostate leucinezipper，PRISE）基因是新近发现的与前列腺癌高度相关的新基因。初步研究提示其高表达与前列腺癌的恶性进展有关。我们的前期研究发现过表达PrLZ可以增强LNCaP细胞的体外生存能力，本研究拟探讨其对LNCaP细胞的体外侵袭潜能的影响及可能的机制。方法：应用Lipofectamine2000将PrLZ重组表达质粒转染至LNCaP细胞，经G418筛选获得稳定高表达PrLZ的LNCaP／PrLZ细胞。Transwell检测PrLZ表达变化对LNCaP细胞体外迁移及侵袭能力的影响。Western blot及明胶酶谱检测基质金属蛋白酶-2（matrixmetalloproteinase-2，MMP-2）的表达水平及活性的变化。结果：迁移实验发现LNCaP与LNCaP／PrLZ细胞穿膜细胞数分别为64.8±8．7与69．3±7．6个细胞／视野，两者相比差异无统计学意义（P〉0．05），而侵袭实验中LNCaP与LNCaP／PrLZ细胞穿膜细胞数分别为70．7±2．8与190．5±9．3个细胞／视野，两者相比差异有统计学意义（P〈0．001），Westernblot证实LNCaP／PrLZ细胞中MMP-2表达水平显著上调，明胶酶谱分析证实LNCaP／PrLZ细胞MMP-2的活性增强。结论：PrLZ在前列腺癌的侵袭过程中可能起-定作用。

英文摘要：

Background and Objective： Prostate leucine zipper （PrLZ）, a novel gene, is highly associated with the malignant progression of prostate cancer. We previously found that PrLZ overexpression can enhance the survival of prostate cancer LNCaP cells in vitro. This study was to investigate the impact of PrLZ on the invasion of LNCaP cells and its possible mechanism. Methods.. PrLZ was transfected into LNCaP cells. LNCaP/PrLZ cells with high expression of PrLZ were screened with G418. The migration and invasion of LNCaP and LNCaP/PrLZ cells were detected by Transwell assay. The level and activity of matrix metalloproteinase-2 （MMP-2） was determined by Western blot and Zymography, respectively. Results： Migration cell count of LNCaP and LNCaP/PrLZ cells were 64.8 ±8.7 and 69.3±7.6, respectively （P〉0.05）, whereas invasive cell count of LNCaP and LNCaP/PrLZ cells were 70.7±2.8 and 190.5±9.3, respectively （P〈0.001）. Both the expression and activity of MMP-2 in LNCaP/PrLZ cells were enhanced. Conclusion： PrLZ may be involved in the invasion of prostate cancer.