中文摘要：

目的探讨使用丁硫氨酸-亚砜胺（L-Buthionine-sulfoximine,BSO）抑制谷胱甘肽（glutathione,GSH）合成对小鼠睾丸支持（TM4）细胞排铅能力的影响。方法体外培养小鼠TM4细胞株,选择对数生长期细胞暴露于浓度为20μmol/L乙酸铅（PbAC）溶液24 h,用GSH合成抑制剂BSO抑制GSH合成,观察低GSH水平下TM4细胞排铅能力变化。采用还原型GSH试剂盒检测各试验组细胞内还原型GSH浓度;采用原子吸收分光光度法检测各排铅时间点（脱离铅环境后第0、1、3、6、9、12 h）细胞内铅残留量,并计算铅相对残留量,以检测细胞排铅能力。结果 GSH检测结果显示,BSO组细胞内GSH水平明显低于对照组,BSO（25）组与溶剂对照组比较差异有统计学意义（P=0.000）,PbAc（20）＋BSO（25）组与PbAc（20）组比较差异有统计学意义（P=0.001）;排铅试验结果显示,在同一排铅时间点,BSO组与对照组细胞内铅相对残留量不同,BSO组在排铅时间点第1、3、6、9、12 h铅相对残留量均高于对照组,差异有统计学意义（P〈0.05）。结论 BSO能抑制TM4细胞合成GSH,细胞内GSH含量不足会降低细胞内重金属铅的外排转运。

英文摘要：

Objective To explore the effects of lead discharge ability with GSH synthesis inhibition by BSO in testicle cells of mice.Methods TM4 cells were exposed to 20ìmol / L concentrations of lead acetate for 24 hours,after that BSO was used to inhibit GSH synthesis in TM4 cells,and the change of lead discharge ability was observed. The reduced GSH level were measured by GSH kit. the lead residue in the cells of different excretion time points（ at 0th、1th、3th、6th、9th、12th hour after departing from the lead environment） was determined by atomic absorption spectrum（ AAS）,and lead residue was measured to test the ability of excluding lead. Results The reduced GSH level of BSO（ 25） groups were lower than that of control groups（ P = 0. 000）,PbAc（ 20） ＋ BSO（ 25） groups were lower than that of control groups（ P = 0. 001） as well. AAS test results showed that the lead residue of BSO（ 25）groups were more than control groups at all the excretion time points（ 1th、3th、6th、9th、12th hours after departing from the lead environment）（ P 〈0. 05）. Conclusion BSO can inhibit GSH synthesis and reduce lead excretion in testicle cells of mice.