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中文摘要：
目的研究半合成β-1，4-葡聚糖硫酸钠（Na—MCS）的抗凝血效果和作用机制。方法采用凝固分析法、以肝素为阳性对照进行抗凝血效果的评价，再通过发色底物法对凝血因子进行抑制分析，揭示Na—MCS的抗凝血作用机制。结果0．6μg·mL^-1Na—MCS即可显著延长APTT和TT，但在更高浓度下对PT影响仍不大，使正常人血浆的APTT延长1倍的Na—MCS剂量为0．7μg·mL^-1，低于活性为150u·mg^-1的肝素。结论在一定浓度范围内，Na-MCS的抗凝血活性与肝素相当，Na—MCS通过抗凝血酶AT—Ⅲ的调节作用来抑制凝血因子IIa和Xa的活性，从而产生抗凝血作用。
英文摘要：
Aim To investigate the anticoagulant efficacy and mechanism of a semi-synthesized sodium β-1,4-glucan sulfate （Na-MCS）. Methods Anticoagulant activity was evaluated by means of coagulation assays in comparison with heparin. The anticoagulant mechanism of Na-MCS was disclosed by inhibitory analysis of the activities of coagulation factors using chromogenic substrates. Results 0. 6 μg·mL^-1 Na-MCS could significantly prolong APTT and TT, but has less effect on PT at an even higher concentration. The dosage of Na-MCS required to double APTT of normal human plasma was 0. 7 μg·mL^-1, lower than that of heparin with the activity of 150 u·mg^-1 Conclusion Na-MCS represented a potent anticoagulation activity in vitro, which matched the efficacy of heparin in a certain range of concentrations. Na-MCS exhibited anticoagulant activity due to inhibition of the coagulation factors IIa and Xa by the mediation of anti-thrombin AT-Ⅲ.
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