中文摘要：

目的研究不同自酸蚀粘接剂牙本质粘接的耐久性，以期为临床应用提供参考。方法选择人离体磨牙42颗，制备拾面牙本质平面试件（共42个试件），分别使用两步法自酸蚀粘接剂A（ClearfilSEBond）和一步法自酸蚀粘接剂B（AdperPrompt）、C（G-Bond）（每种粘接剂分别为14个试件），用复合树脂形成树脂冠，将试件在粘接后24h切割成片状或条状试样。切割后即刻或水浸泡6个月后测试微拉伸粘接强度，扫描电镜下观察粘接界面的纳米渗漏情况。结果粘接24h切割后即刻粘接剂A的粘接强度[（40．60±5．76）MPa]显著高于粘接剂B[（19．06±1．50）MPa]和C[（17．75±1．10）MPa]（P〈0．05）。水浸泡6个月后，粘接剂A的粘接强度[（36．04±3．15）MPa]低于切割后即刻，但差异无统计学意义（P〉0．05），粘接剂B[（11．19±1．97）MPa]和C[（9．14±1．15）MPa]的粘接强度均显著低于切割后即刻（P〈0．05），粘接剂A的强度仍显著高于粘接剂B和C（P〈0．05）。纳米渗漏结果显示，粘接剂B和C的界面渗漏较粘接剂A更显著。结论体外长期水浸泡保存有可能对自酸蚀粘接剂界面的稳定性有影响，一步法自酸蚀粘接剂较两步法更为明显。

英文摘要：

Objective To investigate the durability of self-etch adhesives bonded to dentin in vitro. Methods Forty-two extracted human molars were selected and oeclusal dentin surfaces were exposed. The teeth were randomly distributed into three groups based on adhesives applied. The one-step self-etch adhesive B（ Adper Prompt） and C（G-Bond） and two-step self-etch adhesive A （Clearfil SE bond） were used. After application of the adhesives to the dentin surfaces, composite crowns were built up, after 24 h water storage, the teeth were sectioned longitudinally into sticks（ 1.0 mm × 1.0 mm bonding area） for microtensile testing or slabs （ 1 mm thick） for scanning electron microscopec （ SEM ） observation. Bonding strength（mTBS） and nano-leakage were evaluated immediately after cutting or after 6 months in water. The mTBS was analyzed using one-way ANOVA（ SPSS 13. 0）. The nanoleakage was observed by SEM with a backscattered electron detector. Results Both adhesives and water storage time affected the mTBS. All adhesives showed decreased bond strength after six-month water aging [ A dropped from （40. 60 ± 5.76） MPato （36.04 ±3.15） MPa; B dropped from （19.06±1.50） MPato （11.19±1.97） MPa; C dropped from （ 17.75 ± 1.10） MPa to （9.14 ± 1.15） MPa] （P 〈0. 05）. B and C showed lower mTBS than A after aging （P 〈 0.05） . Compared to A, nanoleakage was more obvious after aging for B and C. Conclusions All self-etch adhesives tested were probably influenced by water aging, however, the two-step adhesive showed better durability than the one-step adhesives.