中文摘要：

目的：分析miR-200a及AFP在肝癌、肝硬化患者血清中的表达水平并进行比较,探索其成为肝癌早期诊断血清标志物的可能性。方法：临床收集肝正常、肝硬化、肝癌患者血液标本。运用实时定量PCR技术检测血清miR-200a的相对表达情况,血清AFP水平从临床资料中提取。结果：临床标本分析结果显示,miR-200a在肝硬化及肝癌患者中均显著下调（P〈0.05）,AFP仅在肝癌患者中出现异常表达。结论：血清miR-200a极大程度地参与了肝癌发生,对肝硬化及肝癌具有一定的诊断价值。

英文摘要：

Objective：This study aimed to analyze the expression levels of serum microRNA-200a（miR-200a） and AFP in liver cirrhosis and liver cancer patients,and to explore whether miR-200a could be a novel biomarker of liver cancer.Methods： Patients with normal liver,liver cirrhosis and liver cancer in clinic were included in this study.Blood samples of the patients were collected for analysis.Real time quantitative PCR technique was used to measure the expression level of serum miR-200a.The AFP expression was got from clinical data.Results： Comparing with normal liver patients,miR-200a presented lower expressions in liver cirrhosis patients and liver cancer patients（P〈0.05）,while AFP presented higher expressions only in liver cancer patients（P〈0.05）.Conclusion： Data indicated that MiR-200a played an important role in liver cancer development and had diagnostic value for indicating liver cirrhosis and liver cancer.