中文摘要：

beta_(1-40 ) 的 oligomers 在 PBS 缓冲液的肽被秒和本国的页分析，并且 Ap ^ o 更整齐被秒也孤立。另外，可溶的 A 的效果 beta_(1-40 ) 在细胞内部的免费的钙(Ca~(2+)) 上更整齐出生后的老鼠的海马趾的神经原的平衡被荧光显微镜学调查。试验性的结果显示beta_( 1-40 )肽在 0.231mmol/L 在低分子量 oligomers 形式存在新鲜 A beta_( 1-40 )答案( 20 mmol/L 磷酸钠缓冲区，酸碱值 7.4 ，0.02%叠氮化钠)在 24 h 以内并且可溶更整齐是最丰富的种类。三聚的和纤丝状的 A beta_(1-40 ) 能增加细胞内部的 Ca~(2+) 集中，而是 Abeta_(1-40 ) 更整齐引起了渐渐的上升，潜力也是比在一样的集中的纤丝的强壮的。另外为三聚的和纤丝状的 A beta_(1-40 ) 有不同反应模式。有 intracellularCa~(2+) 的增加的不同机制的意思由 beta_(1-40 ) 引起了。

英文摘要：

The oligomers of Aβ（1-40）peptide in PBS buffer solution were analyzed by SEC and native PAGE, and the trimer of Aβ（1-40） was also isolated by SEC. In addition, the effects of the soluble Aβ（1-40） trimer on intracellular free calcium （Ca^2＋） balance of hippocampal neurons of postnatal rats were investigated by fluorescence microscopy. The experimental results indicated that Aβ（1-40） peptide existed in the form of low molecular weight oligomers in 0.231 mmol/L fresh Aβ（1-40） solution （20 mmol/L sodium phosphate buffer, pH 7.4, 0.02% sodium azide） within 24 h and the soluble trimer was the most abundant species. Both the trimeric and the fibrillar Aβ（1-40） were able to increase the intracellular Ca^2＋ concentration, but the Aβ（1-40） trimer caused a gradual rise and the potential was also stronger than that of the fibrils at the same concentration. In addition there were different response modes for trimeric and fibrillar Aβ（1-40）. meaning that there are different mechanisms of increase in intracellular Ca^2＋ caused by Aβ（1-40）.