中文摘要：

目的：检测支原体单克隆抗体PD4、黏附分子CD44和增殖细胞核抗原PCNA蛋白在人喉癌组织中的表达,探讨其在人喉癌发生、发展及预后中的作用。方法：采用免疫组织化学Envision法检测214例喉不同病变组织标本（喉癌140例,喉癌前病变25例,声带息肉36例和癌周正常喉组织13例）中PD4、CD44及PCNA蛋白的表达。结果：①PD4、CD44、PCNA在人喉癌组织中表达阳性率分别为45.71%（64/140）、64.29%（90/140）、77.86%（109/140）,明显高于喉其他不同病变组织（P〈0.01）;②PD4、CD44阳性表达率在中晚期（Ⅲ-Ⅳ期）喉癌组织中均显著高于早期（Ⅰ-Ⅱ期）喉癌组织,在伴颈淋巴结转移的喉癌组中阳性表达率均显著高于无颈淋巴结转移的喉癌组,喉癌术后3年或5年存活病例表达阳性率明显低于未存活病例（P〈0.01或P〈0.05）;③PD4、CD44、PCNA在人喉癌组织中联合表达阳性率为27.86%（39/140）,显著高于喉其他不同病变组织中联合表达阳性率（5.41%,P〈0.01）。结论：PD4、CD44及PCNA在喉癌组织中的高表达与人喉癌的发生、发展及预后有一定的相关性。

英文摘要：

Objective：To study the expression of PD4、CD 44 and PCNA proteins in laryngeal carcinoma and their relationships with the patho genesis, development and prognosis of laryngeal carcinoma.Method：Immunohistochemistry was used to study 140 cases of laryngeal carcinoma tis sues,25 cases of precarcinoma tissues,36 cases of vocal cord polyps and 13 cases of normal tissues adjacent to laryngeal carcinoma.Result：① The positive rates of PD4,CD44 and PCNA were 45.71%（64/140）,64.29%（90/140） and 77.86%（109/140） in laryngeal carcinoma,which were much higher than in non-carc inoma tissues（P0.01）.②The third and fourth stages laryngeal carcinoma exp ress stronger PD4 and CD44 than those of the first and second stages.Laryngeal carcinoma with cervical metastasis had higher expression than those without cerv ical metastasis.To 3 and 5 years＇ survival,PD4、CD44 and PCNA positive cases ha d lower chance than those negative cases（P0.01 or 0.05）.③The over all pos itive rate of PD4、CD44 and PCNA was 27.86%（39/140） in laryngeal carcinoma tissu es and 5.41%（4/74） in non-carcinoma tissues.Conclusion：The high expression of PD4,CD44 and PCNA proteins maybe closely related to the path ogenesis, development and prognosis of laryngeal carcinoma.