中文摘要：

目的：研究去卵巢及辛伐他汀（simvastatin,SVS）对大鼠骨痂骨保护素（osteoprotegerin,OPG）表达的影响。方法：2月龄雌性SD大鼠采用去卵巢（ovariectomy,OVX）方法模拟人绝经后骨质疏松症,造模成功后进一步建立胫骨骨折髓内钉内固定模型。大鼠分为3组：假手术组（sham＋vehicle）、空白组（OVX＋vehicle）及实验组（OVX＋SVS）。OVX＋SVS组于骨折端皮下注射辛伐他汀（10 mg.kg-1.d-1×5 d）,sham＋vehicle及OVX＋ve-hicle组仅注射无辛伐他汀的空白溶剂。骨折后1、2、4周取骨痂标本,切片免疫组化染色,分析骨痂中的增殖细胞核抗原（PCNA）、OPG表达情况。结果：结果显示OVX＋vehicle组骨折后1、2、4周PCNA阳性率较sham＋vehicle组分别下降17.3%、32.1%和26.1%（P〈0.01）;OVX＋SVS组骨折后1、2、4周PCNA阳性率较OVX＋vehicle组分别增加60.1%、67.7%和67.7%（P〈0.01）;半定量结果显示OVX＋vehicle组OPG表达水平最低,sham＋vehicle组最高,而OVX＋SVS组的OPG表达水平介于2组之间,OVX＋SVS组与OVX＋vehicle组间差异显著（P〈0.01）。结论：本实验显示去势可明显减少骨痂OPG的表达,而辛伐他汀可刺激骨痂分泌OPG,提示他汀类药物具有间接抑制破骨细胞的作用。

英文摘要：

AIM： To investigate the effect of ovariectomy（OVX） and simvastatin（SVS） on the expression of osteoprotegerin（OPG） in bone callus of rats.METHODS： Simvastatin（10 mg·kg-1·d-1） was injected subcutaneously to tissue overlying the site of fractured tibiae of ovariectomized rats for a treatment period of 5 days.Vehicle reagent was used as control in sham and OVX rats.Bone callus were harvested at time points of 1,2 and 4 weeks after fracture and the expression of proliferating cell nuclear antigen（PCNA） and OPG was measured by the method of immunohistochemistry.RESULTS： Compared with OVX＋vehicle group,the expression of PCNA decreased by 17.3%,32.1% and 26.1% in OVX＋vehicle group than that in sham＋vehicle group（P0.01）,and increased by 60.1%,67.7% and 67.7% in OVX＋SVS group（P0.01） at the time points of 1,2 and 4 weeks after fracture,respectively.The expression of OPG was the lowest in OVX＋vehicle group.The significant differences of OPG expression between OVX＋SVS group and OVX＋vehicle group at the 3 time points（P0.01）,and significant differences of OPG expression between sham＋vehicle group and OVX＋vehicle group at 1st week and 2nd week after fracture（P0.05） were observed.CONCLUSION： Ovariectomy decreases the expression of OPG in bone callus of rats,while simvastatin increases it,indicating that simvastatin can indirectly inhibit the function of osteoclasts.