中文摘要：

目的 克隆白纹伊蚊唾液腺黏蛋白相关蛋白基因1（Aamucin1）的全长cDNA序列，并分析白纹伊蚊吸血前后基因表达的差异。 方法 根据白纹伊蚊罗马株Aamucin1基因序列（GenBank序列号为AY826068）设计特异引物，提取白纹伊蚊广州株唾液腺总RNA，PCR扩增Aamucin1全长基因序列，并进行生物信息学分析。实时荧光定量RT-PCR分析白纹伊蚊吸血前后Aamucin1基因表达差异。 结果 成功克隆白纹伊蚊广州株基因，并获得全长为849 bp的基因序列，编码283个氨基酸。白纹伊蚊广州株与罗马株Aamucin1基因序列比对在C端少了13个氨基酸。与罗马伊蚊同源序列的一致性为58%；该基因具有1个可变剪接子；Protscan分析示有苏氨酸丰富区，可变剪接子恰位于此区；饱血零时（吸血2 h，腹部饱胀者，为BSG_0组），Aamucin1基因表达显著下调，是吸血前（SG组）的0.39倍（P〈0.01）；饱血24 h（饱血后24 h，为BSG_24组），Aamucin1基因表达较饱血零时有回升，是吸血前（SG组）的0.61倍（P〉0.05）。 结论 获得白纹伊蚊广州株Aamucin1基因全长序列，并证实其在mRNA水平受到吸血调控。 Service

英文摘要：

Objective To clone the mucin-related protein（Aamucin1）gene from salivary gland of Aedes albopictus Guangzhou isolate, and analyze the expression difference due to blood-feeding. Methods Total RNA was extracted from the salivary gland. The coding region of Aamucin1 was amplified with a pair of specific primers by RT-PCR. The product was sequenced and analyzed by bioinformatics. Expression analysis was conducted by real-time RT-PCR. Results The product of RT-PCR was 849 bp with encoding 283 amino acids. To compare with that from Ae. albopictus Rome strain, 13 amino acids were deleted at the C end, and Aamucin1 in Guangzhou isolate shared 58% identity in amino acids with that of Rome isolate. In addition, an alternative splicing was found in Aamucin1 and located in a proline enrich area by Protscan. To compare with that of non-blood-feeding（group SG）, Aamucin1 was significantly down-regulated with 0.39 fold expression at zero time after engorged （group BSG_0, mosquitoes with abdominal distention from the first 2 hours after blood-feeding, P〈0.01） and 0.61 fold expression at the 24th hour after engorged（group BSG_24,mosquitoes from the 24th hours after blood-feeding, P〉005）. Conclusion The full length of Aamucin1 gene of Ae. albopictus is cloned and it can be modulated by blood-feeding.