运用生物膜反应器(BAR)模拟实际原水输水管道,研究了管道运行时间对生物膜微生物群落结构及出水微污染物浓度的影响。结果表明,随着BAR反应器运行时间的延长,出水pH值和CODMn浓度变化较小,溶解氧、氨氮和总氮浓度逐渐降低,运行180d时,对氨氮和总氮的去除率最高,分别为41.3%和30.3%,浊度呈曲线变化;采用454高通量测序对运行90、120、180和240d的四个生物膜样品中微生物群落结构进行分析,发现其微生物群落结构存在较大差异,90d的生物膜的微生物多样性最高,而240d的生物膜具有最高的微生物丰度;BAR系统水质影响生物膜微生物的群落结构,同样,生物膜中的微生物净化了出水。
Biofilm annular reactors (BARs) were used for simulating practical raw water distribution pipeline, and the effect of operation time on the biofilm microbial community structure and the concentration value and of pollutants in the outlet wa CODM. concentration slightly ter was studied. The results indicated that in the outlet water, pH changed, the dissolved oxygen ( DO), ammonia nitrogen and total nitrogen gradually reduced, the highest removal rate of ammonia nitrogen and total nitrogen occurred in the biofilm for 180 d, which were respectively 41.3% and 30.3%, and the turbidity unsteadily varied. The analysis of 454 high-throughput pyrosequencing showed that there was obvious difference in biofilm microbial community structure among the four biofilm samples which operated for 90 d, 120 d, 180 d and 240 d respectively. The highest microbial diversity was found in the biofilm for 90 d, and the highest richness was detected in the biofilm for 240 d. The biofilm microbial community structure was influenced by water quality. Likewise, the outlet water was purified by the microorganisms in the biofilm.