中文摘要：

目的制备罗哌卡因幼鼠毒性惊厥模型，观察毒性惊厥对幼鼠学习记忆能力和海马突触素表达的影响。方法选用21日龄SD鼠60只，按照随机数字表法分为罗哌卡因和生理盐水组（每组30只）。2组各取20只并根据不同取材时间点又分为注射后24h、3d、7d、60d4个亚组（每组5只），选取幼鼠海马组织，采用Western blotting行突触素蛋白含量测定；剩余各组10只于上述时间点进行水迷宫寻找平台潜伏期测试。结果罗哌卡因组幼鼠惊厥后寻找平台潜伏期随时间延长逐渐缩短．各时间点比较差异有统计学意义（P〈0．05）。生理盐水组幼鼠惊厥后各时间点潜伏期比较差异无统计学意义（P〉0．05）。罗哌卡因组24h、3d时寻找平台潜伏期分别为（38．62±19．08）S、（18．40±7．95）S，明显长于同时期生理盐水组[（13．08±4．73）S、（14．17±3．28）S]，差异有统计学意义（P〈0．05）。罗哌卡因组幼鼠注射后24h突触素蛋白表达为O．25±0．03，生理盐水组为0．34±0．03，比较差异有统计学意义（P〈0．05）。结论幼鼠单次罗哌卡冈毒性惊厥对其学习记忆存在一过性的影响．推测与突触素蛋白表达有关。

英文摘要：

Objective To study the effect of ropivacaine-induced toxicity on learning and memory abilities and synaptophysin expression of the hippocampus in immature mice. Methods Sixty 21-d-old SD mice were randomly divided into ropivaeaine inducement group （R, n=30） and sodium chloride treatment group （N, n=30）. Mice in each group were subdivided into 4 subgroups according to the different injection times （24 h, and 3, 7 and 60 d after convulsion or injection, n=5）; the protein expression of synaptophysin in the hippocampus at each time point were detected by Western blotting. The left 10 mice in each group were performed Mirror water maze test to explore the latency in finding the platform. Results The latency in finding the platform in mice of group R was gradually shortened as time being prolonged, and significant difference was noted between each 2 time points （P〈0.05）; while no significant difference was noted between each 2 time points in mice of group N （P〉0.05）. The latency in finding the platform in mice of group R was obviously longer as compared with that in group N at 24 h and 3 d time point （P〈0.05）, but there were no significant differences at other time points （P〉0.05）. The synaptophysin expression in the hippocampus of mice in group R was significantly lower as compared with that in group N at 24 h time point （P〈0.05）. Conclusion The effect of single ropivacaine toxicity on learning and memory impairment of immature mice is transitional, which might be correlated to the synaptophysin expression in the hippocamous.