中文摘要：

目的测定铜绿假单胞菌噬菌体PaP3的最佳感染复数、一步生长曲线和吸附K值及交叉吸附K值等基本生物学特性。方法按照感染复数（MOI）分别为0．0001、0．001、0．01、0．1、1和10加入噬菌体纯培养液和宿主菌，充分裂解细菌后，测定噬菌体滴度；以MOI=10的比例加入噬菌体及宿主菌，进行一步生长实验；纯化PaP3颗粒，免疫家兔，获得抗血清，通过中和反应实验测定PaP3和其抗血清之间的吸附反应常数K值。同时，利用抗血清交叉中和试验确定本室分离的三株噬菌体之间的血清学关系。结果当MOI=0．001时PaP3感染其宿主菌产生的子代噬菌体滴度最高；根据一步生长实验结果绘制一步生长曲线；通过血清交叉中和反应得出不同的吸附常数。结论PaP3最佳感染复数为0．001，感染宿主菌的潜伏期是20min，爆发期是60min，平均爆发量约为31，其抗血清反应的吸附常数K值为262。

英文摘要：

Objective To identify some basic biological properties,optimal multiplicity of infection（MOI） ,one-step growth kinetics,serum neutralization rate constant of Pseudomonas aeruginosa phage PaP3 were determined, respectively. Methods The host bacteria were infected with PaP3 at six different MOI（0. 0001,0. 001,0. 01,0. 1,1 and 10,then lysated adequately, the phage titer of superuatant was determined. For one-step growth experiments,the host bacteria were infected with PaP3 at MOI= 10, following centrifugation after 15 min adsorption,the pellet containing （partially） infected cells was resuspended in 3 ml of pre-warmed I.B broth and incubated with 160rpm at 37℃. Samples（50pd） were taken at 10 min-intervals and immediately tittered by the double-layer agar plate method. A rabbit was immunized with purified PaP3 to prepare anti-PaP3 serum, reaction constants between antiserum and PAP1, PAP2, PaP3 were determined using cross neutralization test. Results 0. 001 MOI-infected host bacteria gave the highest phage offsprings. One-step growth curve was determined according to the results of one-step growth experiments. Reaction constant was determined using neutralization test. Conclusion The optimal MOI of PaP3 was determined to be 0. 001. One-step growth curve for PaP3 shows that the latent period is about 20 rain, the rise period is 60 rain, and the average burst size is about 31pfu/cell. Rate constant of reaction between antiserum and PaP3 is 262.