中文摘要：

目的探讨微小RNA-34a（miR-34a）在碘乙酸钠所致大鼠膝骨关节炎模型中的表达变化，并初步明确其功能。方法选取30只雄性Wistar大鼠，左侧膝盖不注射碘乙酸钠作为对照组，右侧膝盖注射碘乙酸钠作为模型组；造模完成后检测两组大鼠关节液内miR-34a表达水平的变化；分离两组大鼠的膝关节软骨细胞并进行原代培养，敲低miR-34a后，使用流式细胞仪测定细胞凋亡情况。结果模型组Makin评分为（5．09±1．35）分，对照组为（1．27±0．64）分，差异有统计学意义（P〈0．05）。与对照组比较，模型组中miR-34a在关节液和关节软骨细胞内的、表达均升高，差异均有统计学意义（P〈0．05）。将miR-34a用它的反义互补片段敲低后，模型组大鼠关节软骨细胞的凋亡受到明显抑制（P〈O。05）。结论miR-34a在碘乙酸钠所致犬鼠膝骨关节炎的关节液中表达升高，并且抑制miR-34a的表达会降低关节炎大鼠的关节软骨细胞凋亡率。

英文摘要：

Objective To explore the expression of microRNA-34a（miR-34a） in sodium iodoacetate-induced rat model of os- teoarthritis rat model and to initially clarify its function. Methods Thirty male Wistar rats were selected. The right knees were in- jected with sodium iodoacetate （model group） and the left knees served as the control group without injecting by sodium iodoace- tate. The expression levels of miR-34a in the synovial fluid and articular chondrocytes were compared between two groups after con- structing the model. Articular chondrocytes in the two groups were separated for conducting the primary culture, after miR-34a knockdown,the flow cytometry was used to determine cell apoptosis. Results The Makin score in the model group was（5.09-＋- 1.35） points, which was （1.27 ~ 0. 64） points in the control group, the difference had statistical significance （P〈 0.05）. Compared with the control group, the expression levels of miR-34a in the synovial fluid and articular chondrocytes in the model group were in- creased,the difference was statistically significant（P〈0.05）. After the miR-34a knockdown by using its complementary antisense fragment,articular chondrocytes apoptosis in the model group was significantly inhibited（P〈0.05）. Conclusion The expression of miR-34a in the synovial fluid of sodium iodoacetate-induced knee osteoarthritis is increased,moreover inhibiting the microRNA-34a expression could reduce the apoptotic rate＇ of articular chondrocytes in osteoarthritis rat.