量点的光致发光性质被环境强烈总是影响的事实在 QD 简历申请的地里限制进一步的进步的范围。在这份报纸,(丙烯酸的酸)( 泛美航空公司) 光致发光性质和与 amphiphilic 涂的水溶性的 CdSe/ZnS 核心壳量点的稳定性上的免疫球蛋白 G (IgG ) 的效果 poly 被学习。光致发光( PL )系列,紫外力的系列和激动的州的一生大小被用来描绘不同蛋白质分子的影响,例如 IgG (山羊反人的 IgG ,兔子反人的IgG人的 IgG 结合的反人的 IgG ,人的 IgG ,和山羊), QD 的 PL 性质上的卵白素和牛的浆液白朊( BSA )。当 IgG 分子被增加进 QD 答案时, CdSe/ZnS 的 PL 紧张和稳定性大部分与纯 CdSe/ZnS QD 的相比被提高。PL 紧张与增加 IgG 集中增加,但是出现对在半最大值(FWHM ) 的 PL 山峰和完整的宽度的没有影响。不同蛋白质分子的功能取决于蛋白质分子的结构的 QD 的 PL 进化,它被用作一个传感器认出人的 IgG。在泛美航空公司涂层层和 IgG 分子之间的相互作用导致 PL 紧张的改进,这被推断。QD-IgG 的光性质上的 pH 和离子力量的效果的学习与纯 QD 答案相比,混合答案建议 pH 价值和离子力量不破坏在泛美航空公司涂层层和 IgG 之间的相互作用。激动的州的一生分析显示 PL 改进与 PAA 涂层层来自 QD 的表面的钝化。IgG 分子没在生物系统的性质上有效果,但是能增加稳定性和 CdSe/ZnS QD 的 PL 紧张,它将在 biomedicine 和另外的领域扩大 QD 的应用程序。
The fact that the photoluminescence properties of quantum dots are always strongly influenced by the environment limits the scope of further progress in the field of QD' bio-applications. In this paper, the effects of immunoglobulin G (IgG) on the photo- luminescence properties and stability of water-soluble CdSe/ZnS core-shell quantum dots coated with amphiphilic poly (acrylic acid) (PAA) are studied. Photoluminescence (PL) spectra, UV-vis spectra and excited state lifetime measurements are used to characterize the influence of different protein molecules, such as IgG (goat anti-human IgG, rabbit anti-human IgG, human IgG, and goat anti-human IgG-human IgG conjugates), avidin and bovine serum albumin (BSA) on the PL properties of QDs. The PL intensity and stability of CdSe/ZnS are largely enhanced compared to that of pure CdSe/ZnS QDs when the IgG molecules are added into the QD solution. The PL intensity increases with increasing the IgG concentration, but there appears no influence on the PL peak and a full width at half maximum (FWHM). The PL evolution of QDs as a function of different protein molecules depends on the structure of protein molecules, which is used as a sensor to recognize human IgG. It is inferred that the interaction between PAA coating layer and IgG molecules results in the enhancement of PL intensity. The study of the effect of pH and ion strength on optical properties of QD-IgG mixed solution, compared with the pure QD solution, suggests that pH value and ion strength do not destroy the interaction between the PAA coating layer and IgG. Excited state lifetime analysis indicates that the PL enhancement comes from the passivation of surface of the QDs with the PAA coating layer. IgG molecules have no effects on the properties of the biological system but can increase the stability and PL intensity of CdSe/ZnS QDs, which will enlarge the application of QDs in biomedicine and other fields.