中文摘要：

目的通过沉默系统性红斑狼疮（SLE）患者骨髓间充质干细胞（MSC）Olf-1／EBF相关锌指蛋白（OAZ）基因，探讨OAZ基因异常表达对MSC功能的影响。方法收集培养5例SLE患者及5名健康对照者骨髓MSC，以实时荧光定量聚合酶链反应（PCR）检测OAZ基因在SLE患者与健康对照组间差异；再收集培养6例SLE患者骨髓MSC，运用Aeeell技术沉默OAZ基因，以实时荧光定量PCR检测OAZ、分化抑制因子（Id）1-3及CC趋化因子配体2（CCL2）的mRNA表达水平，收集共培养上清，用酶联免疫吸附试验（ELISA）检测CCL2的表达。采用t检验或Mann．Whitney秩和检验进行统计分析。结果①SLE患者MSC中OAZ基因表达量（0．013±0．016）显著高于健康对照组（0．001±0．000，P=0．009）。②OAZ基因沉默组OAZ、Id1、Id2、Id3基因mRNA表达水平（ACt分别为10．3±0．7，15．2±1．6，8．1±1．4，10．5±0．6）显著低于阴性对照组（ACt分别为8．7±0．7，14．1±1．2，7．1±1．5，9．8±0．6）（P均〈0．05）。③OAZ基因沉默组CCL2mRNA表达水平（ACt为2．2±1．1）显著高于阴性对照组（ACt为3．0±1．1）；MSC培养上清中，OAZ基因沉默组CCL2表达水平（341±29）pg／ml显著高于阴性对照组（304±19）pg／ml（P均〈0．05）。结论OAZ基因在SLE患者MSC中高表达；OAZ基因可以调控MSC中CCL2的表达，这一效应可能在SLE自身抗体产生中发挥作用。

英文摘要：

Objective To study the role of~ Olf-1/EBF associated zinc finger protein （OAZ）, a trans- cription factor encoded by a positional systemic lupus erythematosus （SLE） candidate gene, in the function of mesenchymal stem cells （MSC） of SLE patients by silencing this gene. Methods OAZ mRNA levels of bone marrow MSC obtained from 5 SLE patients and 5 healthy controls were detected by real-time PCR. Bone marrow MSC obtained from 6 SLE patients were incubated with specific siRNAs for 3 days, then cells were harvested for OAZ measurement, Idl-3 and CCL2 mRNA levels were tested by real-time PCR, and levels of CCL2 were detected in culture supernatants using ELISA. Differences between groups were analyzed using t-test or Mann- Whitney test. Results （1） OAZ mRNA levels of bone marrow MSC were significantly elevated in SLE patients （0.013±0.016） compared to healthy controls （0.001±0.000, P=-0.009）. （2 After OAZ silencing, the expression levels of OAZ, Idl, Id2 and Id3 mRNA were significantly decreased （ACt 10.3±.7, 15.2±.6, 8.1±1.4, 10.5±.6 vs 8.7±0.7, 14.1±.2, 7.1±1.5, 9.8±0.6） （P all 〈0.05）3 Both the expression levels of CCL2 mRNA （ACt 2.2±.1 vs 3.0±1.1 ） and the levels of CCL2 protein in culture supernatants [ （341±9） pg/ml vs （304±9） pg/ml] were significantly increased in OAZ silencing group comparing to those in the control group （P all 〈0.05）. Conclusion OAZ gene expression is significantly elevated in bone marrow MSC of SLE patients. OAZ may affect autoantibody production in SLE patients by regulating CCL2 expression.