中文摘要：

目的探讨大鼠凝血因子ⅦEGF1片段与脂多糖（LPS）刺激后的大鼠血管内皮细胞（RAOEC）结合能力。方法将0．1mg／LLPS作用RAOEC2h、4h、6h、18h，应用免疫荧光法检测TF蛋白在RAOEC的表达，通过荧光显微镜和流式细胞仪检测4μmol／L、8μmol／L、16μmol／L的大鼠凝血因子ⅦEGF1多肽与LPS作用后的RAOEC的结合能力。结果0．1mg／L LPS能刺激RAOEC表达组织因子（TF），4h最为明显，4μmol／L、8μmol／L、16μmol／L大鼠凝血因子ⅦEGF1能结合LPS刺激后的RAOEC。结论大鼠凝血因子ⅦEGFl片段能结合LPS刺激后RAOEC。

英文摘要：

Objective To explore the capability of the first epidermal growth factor domain of rat coagulation factor Ⅶ to bind with rate aortic endothelial cell induced by lipopolysaccharide. Methods The expressions of tissue factor on RAOEC induced by 0.1 mg/1 LPS for 2,4,6,18 hours were examined by immunofluorescence method. The capability of binding for EGF 1 with TF were determined by fluorescence microscope and flowcytometry. Results TF is expressed on RAOEC induced by 0.1 mg/1 LPS, the effect is the most remarkable after 4h （P 〈0.01 ）,compared with that of control. 4μmol/L,8 μmol/L, 16 μmol/L EGF 1 peptide can bind with the RAOEC induced by LPS. Conclusion The first epidermal growth factor domain of rat coagulation factor Ⅶ to bind with rate aortic endothelial cell induced by lipopolysaccharide.