中文摘要：

目的探讨共培养诱导骨髓问充质干细胞（BMSCs）向睾丸间质细胞（Lc）分化的可行性，为解决组织工程化雄激素分泌组织研究中种子细胞来源不足的问题提供有效手段。方法通过Ficoll液分离、贴壁后传代并富集3周大鼠的BMSCs，及以差速贴壁法获得的大鼠Lc。将两种细胞通过tran-swell培养皿的间接共培养作为实验组，单纯的LC及BMSCs培养组分别做阳性及阴性对照。4周后，各组BMSCs进行Lc的特异性指标免疫组化及RT-PCR检测。结果4周后，免疫组化显示，实验组部分BMSCs表达LC特异性标志物3B-HSD、LHR；RT-PCR检测stAR及3p-HSDmRNA呈阳性表达，而对照组呈阴性。结论通过体外共培养模拟LC生长的微环境，能够诱导BMSCs向LC方向上分化，在改善培养条件及提高诱导效率后，将可能为雄激素缺乏性疾病提供一条有前景的治疗途径。

英文摘要：

Objective To investigate the possibility of inducing the Bone mesenchymal stem cells （BMSCs） to differentiate into leydig cells （LC） by co-culture, providing a new source of seed cells for the tis- sue research of tissue-engineered androgen secretion. Methods LC and BMSCs from 3-week rats were obtained by differential adhesion and Ficoll isolation, the cells were co-cultured with transwell insert equipments as the ex- perimental group. LC or BMSCs with the same cell number were cultured respectively as the control groups. Im- munohistochemistry was applied to determine the expression of 3 [3-HSD and LHR at 4 weeks after co-culture. RT-PCR was adopted to detect the expression of LC. Results At 4 weeks after co-culture, BMSCs in the experi- mental group showed the expression of 3[3-HSD,LHR, the specific makers of the LC by immunohistochemistry, and positive expression of stAR,3[3-HSD by RT-PCR detection. In contrast, the positive ceils were not tested in the control groups. Conclu sion BMSCs can be induced to differentiate into LC by coculture system in vitro. In the further studies, the supply of growth factors or neutrition materials which were needed during LC differentia- tion can improve the efficacy. There will be a safe and effective way to ~btain the proper seed cells for tissue-engi- neered androgen secretion, and may provide a promising method for the diseases of deficiency of testerone.