在0.01mol/LH2SO。的介质中,有聚乙烯醇-124存在时,硫酸奎尼丁(QDS)溶液通过毛细流作用能在憎水性玻璃表面上自组装成环(SOR)。环的最大荧光强度与硫酸奎尼丁的量成正比,据此建立了一种显微荧光自组装成环技术测定血清硫酸奎尼丁的新方法,测定硫酸奎尼丁的线性范围为(0.2~1.4)×10^-12mol;检出限(3σ)为2.6×10^-14mol。本方法已成功用于血清中硫酸奎尼丁的测定,回收率为93.3%-101.3%。
In the sedium of 0.01 mol/L H2SO4 with the aid of poly ( vinyl alcohol)-124, quinidine sulfate (QDS) can form a self-ordered ring (SOR) on the surfaces of hydrophobic glass slides based on the capillary effect. The maximal fluorescence intensity of the ring is proportional to the content of quinidine sulfate. An SOR method for the determination of quinidine sulfate was established in the range of 0.2 - 1.4 × 10 ^-12 tool, and the limit of detection (3σ) was 2.6 × 10^ -14 mol. Determination of quinidine sulfate in serum samples was successfully made with the recovery of 93.3% -101.3%.