中文摘要：

应用高效液相色谱-串联质谱法测定养殖海水中的氟苯尼考残留。通过Shiseido C18色谱柱以甲醇及含0．1％乙酸和10mmol·L^-1乙酸铵的水为流动相进行梯度洗脱。采用电喷雾离子源（ESI），负离子模式，在多反应性监测模式下采集信号，定性离子对为356／185，356／219，356／336，定量离子对为356／336。本方法的线性范围是0．1～100μg·L^-1，其相关系数为0．997，方法检出限为0．01μg·L^-1。该方法快速、灵敏，检测结果准确可靠，适于环境监测。

英文摘要：

An analytical method based on high performance liquid chromatography tandem mass spectrometry （HPLC-MS/MS） has been developed for the determination of florfenicol residues in aquicuhure seawater. The separation was performed on a Shiseido C 18 column with gradient elution using methanol and water （containing 0.1% acetic acid and 10 mmol·L^-1 ammonium acetate）. The spectral acquisition was done in the negative electron spray ionization utilizing multiple reaction monitoring of three parent- daughter ion pairs： m/z 356/185, 356/219 and 356/336. The ion pair of m/z 356/336 was used for quantitation. Linear plots were obtained between 0.1 μg·L^-1 and 100 μg·L^-1, with linear correlation coefficient of 0.997. The limit of detection was 0.01 μg·L^-1. The method is quick, sensitive, reliable and applicable to environmental monitoring program.