中文摘要：

目的：准确定位及尝试剥离大中动物浦肯野纤维密集区.方法：先采用碘染色的方式显示心室传导系统的大致走行,然后用墨汁灌注明确不同动物的心室传导系统浦肯野纤维密集区的位置,在此基础上尝试分离浦肯野纤维密集区组织.结果：牛心墨汁灌注的方法显色清晰、保存持久,而犬心需通过碘染色显色;通过上述2种染色方法,准确定位了牛和犬心室传导系统浦肯野纤维密集区的位置,但准确完整剥离该区却存在较大困难.结论：牛、犬心室内面存在多处浦肯野纤维明显的密集区,这些密集区组织的准确定位,为进一步分离培养浦肯野细胞提供了重要的依据.

英文摘要：

Objective： To accurately localize and separate the Purkinje fiber dense areas in bovine and canine ventricles. Methods： First, iodine dyeing was adopted to show the distribution of the ventricular conduction system roughly. Second, Chinese ink perfusion was used to show the position of Purkinje fiber dense areas. Finally, the Purkinje fiber dense tissue was dissected. Results： Chinese ink perfusion was ideal to bovine Purkinje fiber staining due to its persistence and clarity, while iodine was a better choice for canine Purkinje fiber staining. In our study, the Purkinje fiber dense areas were accurately located through the two dyeing methods. However, it was difficult to accurately and completely separate the fiber tissues. Conclusion： Several Purkinje fiber dense areas have been accurately described. This work may provide an important reference for separating and cultivating cardiac Purkinie cells.