中文摘要：

目的：观察来氟米特（LEF）对MRI/1pr狼疮鼠的疗效及与单核细胞趋化蛋白-1（MCP-1）之间的关系。方法：采用考马斯亮蓝染色、ELISA、HE染色及免疫荧光技术检测尿蛋白、抗ds-DNA抗体、血清肌酐；通过ELISA、免疫组化、Westem B1ot及RT-PCR技术了解LEF组及对照组血清、尿液、肾脏中MCP-1的表达水平。结果：（1）LEF组24h尿蛋白、抗ds-DNA抗体滴度、血肌酐均显著低于对照组，肾小球硬化、间质纤维化程度、炎性细胞浸润、新月体形成率、IgG及IgM沉积较对照组均显著降低；（2）LEF组血清、尿液及肾脏组织中的MCP-1水平较对照组显著降低。结论：LEF通过抑制MCP-1的表达而发挥治疗狼疮性肾炎（LN）的作用。

英文摘要：

Objective：To study To study the effects of leflunomide （LEF）on MRL/lpr mice and monocyte chemoat- tlactant protein-1 （MCP-1） expression. Methods： The curative effect of LEF on MRL/lpr mice was detected by coomassie brilliant blue staining, ELISA, HE staining and immunofluorescence; the expression level of MCP-1 in serum, urine and kidney from the LEF group and the control group were realized by ELISA, immunohistochemistry, western blot and RT- PCR. Results： （1）The 24h urine protein, ds-DNA antibody and serum creatinine in the LEF group were significantly lower than in the control group. The glomerular sclerosis, interstitial fibrosis degree, inflammatory cell infiltration, crescent forma- tion rate, IgG and IgM deposition from the LEF group were obviously depressed in comparison with the control group. （2） The MCP-1 level in serum, urine and kidney from the LEF group were significantly reduced compared with the control group. Conclusions： LEF plays an important role in the treatment of lupus nephritis （LN） by inhibiting the expression of MCP-1.