中文摘要：

目的 探讨大剂量纳米粒径铁粉经口染毒对小鼠血清乳酸脱氢酶（LDH）及其同工酶的影响.方法 40只CD小鼠随机均分（雌雄各半）为对照（Control）组、微米粒径铁粉（Micro-Fe）组、微米粒径氧化铁粉（Micro-Fe2O3）组和纳米粒径铁粉（Nano-Fe）组.将以上材料制成水溶液,以羧甲醛纤维素为辅剂,以5 g/Kg体重剂量一次经口灌胃,14天后处死,分别测定脏器系数,取血分离血清,测定LDH及其同工酶、α-HBDH活性变化.结果 Micro-Fe、Micro-Fe2O3和Nano-Fe组小鼠肝脏系数明显高于对照组（P＜0.05）,血清LDH活性明显低于对照组（P＜0.05）,4组小鼠血清α-HBDH活性间差异无显著性（P＞0.05）.Nano-Fe组小鼠血清LDH3明显低于Micro-Fe组,但与Control组和Micro-Fe2O3组相比差异不显著;Nano-Fe组小鼠血清LDH4和LDH5明显高于对照组（P＜0.05）.结论 大剂量铁摄取可能对小鼠血清LDH具有一定抑制效应,Nano-Fe对血清LDH同工酶的影响与Micro-Fe和Micro-Fe2O3有所不同,血清LDH同工酶分析可能比血清LDH能更好地反映Nano-Fe对生物效应的影响.

英文摘要：

Objective To analyze the effect of excess nano-particle iron on serum α-hydroxybutyrate dehydrogenase （α-HBDH）, lactate dehydrogenase （LDH） and its isoenzyme spectrum in mice. Methods The mice were treated with micro-particle size of iron powder（ M-Fe）, ferric oxide（ M-Fe2O3 ） and nano-particle size iron powder（ NFe）in a single oral dose at 5 g/kg body weight. 14 days later, the mice were sacrificed,the visceral coefficients of heart, liver, kidney and brain were calculated and the serum was collected for α-HBDH, LDH and its isoenzyme spectrum analyzed. Results The visceral coefficient of liver in N-Fe, M-Fe2O and M-Fe groups was higher obviously than that of control group （ P 〈 0.05 ）, without obvious differences （ P 〉 0.05） for serum α-HBDH among 4 groups mice. The serum LDH activity in 3 kinds of iron powder groups mice were markedly lower than that in control group （ P 〈 0.05）, the serum LDH3 content in N-Fe powder mice was lower than that in M-Fe group （ P 〈 0.05）, but without any significant difference compared with control and M-Fe2O3 groups. The serum LDH4 and LDH5contents in N-Fe group mice increased significantly compared with control group. Conclusion Excess iron could probably inhibit serum LDH activity in mice, N-Fe might have some different effects on LDH isoenzyme spectrum compared with micro particle size iron and ferric oxide material. The serum LDH isoenzyme analyses maybe more usefuU than LDH for the evaluation of the effect of excess N-Fe powder.