观察 Xuefu Zhuyu 煎的效果的目的(endothelial 上的琛€搴滈€愮榾姹 ? 包含浆液(XFZYD-CS ) 在 vitro 的祖先房间(EPC ) 试管形成。从老鼠骨头髓的方法 Mononuclear 房间在一台 Ficoll 密度坡度离心机被准备。EPC 被微分附件方法分开,并且在 EPC 试管形成上为 XFZYD-CS 的效果与转换显微镜观察了。在一天以后的结果,暴露于包含 5% , 10% 和 15% 的浆液的 EPC XFZYD-CS 形成了典型试管或容器网络。试管形成时间是在控制组前的二天,在浆液组的大多数试管的尺寸比在控制组小。结论 XFZYD-CS 能导致 EPC angiogenesis 并且赶快试管形成,特别在毛状的容器。学习在 ischemic 疾病的治疗为 Xuefu Zhuyu 煎的似有道理提供试验性的证据。关键词 Xuefu Zhuyu 煎 - endothelial 祖先房间 - 试管形成由中国(号码 30772877 ) 的国家自然科学基础支持了;中国医药科学的中国学院的基本研究资金(没有。ZZ2006039 ) ;综合的药基础(号码 3000-905010805 ) 的福建学院
Objective: To observe the effect of Xuefu Zhuyu Decoction (血府逐瘀汤)-containing serum (XFZYD-CS) on endothelial progenitor cell (EPC) tube formation in vitro. Methods: Mononuclear cells from rat bone marrow were prepared in a Ficoll density gradient centrifuge. EPCs were separated by the differential attachment method, and observed with inverted microscope for the effect of XFZYD-CS on EPC tube formation. Results: After one day, EPCs exposed to the serum containing 5%, 10% and 15% XFZYD-CS formed typical tubes or vessel networks. The tube formation time was two days ahead of the control group and the size of most tubes in the serum groups was smaller than in the control group. Conclusion: XFZYD-CS could induce EPC angiogenesis and hasten tube formation, especially in capillary vessels. The study provides experimental evidence for the plausibility of Xuefu Zhuyu Decoction in the treatment of ischemic diseases.